Vagus nerve stimulation protects against acute liver injury induced by renal ischemia reperfusion via antioxidant stress and anti-inflammation - 31/07/19
, Hong Jiang a, b, c, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 9 |
| Iconographies | 10 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | Clinical evidence has revealed a close relationship between acute liver injury (AKI) and remote organ dysfunction, especially liver injury. |
• | VNS protects against liver injury induced by AKI via anti-inflammation, anti-apoptosis, and enhancing antioxidative capability. |
• | VNS may become a promising adjuvant therapeutic strategy for treating liver injury from AKI in the future. |
Abstract |
Objective |
Renal ischemia reperfusion (I/R) is not an isolated event; however, it results in remote organ dysfunction. Vagus nerve stimulation (VNS) has shown protective effects against renal I/R injury via an anti-inflammatory mechanism. This study aimed to investigate whether VNS could attenuate liver injury induced by renal I/R and identify the underlying mechanisms.
Methods |
Eighteen healthy male Sprague-Dawley rats (200–250 g) were equally divided into three groups: sham group (sham surgery without I/R or VNS), I/R group (renal I/R) and VNS group (renal I/R plus VNS). The I/R model was established by excising the right kidney and then clamping the left renal pedicle with an occlusive nontraumatic microaneurysm clamp for 45 min followed by a 6-h reperfusion. The rats in the VNS group received spontaneous left cervical VNS with renal ischemia and reperfusion. At the end of the experiment, blood and liver tissues were collected to detect liver function, oxidative stress and inflammatory parameters. Additionally, TUNEL staining, real-time PCR, western blotting and hematoxylin and eosin staining of liver tissues were performed to assess liver injury and the underlying mechanisms.
Results |
Kidney and liver function was severely damaged in the I/R group compared to the sham group. However, VNS significantly protected kidney and liver function. Rats treated with VNS revealed decreases in oxidative enzymes, apoptosis and levels of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in serum and liver compared with rats in the I/R group. Rats in the VNS group also showed increased antioxidant stress responses compared to rats in the I/R group.
Conclusion |
VNS exerts protective effects against liver injury from renal I/R via inhibiting oxidative stress and apoptosis, downregulating inflammatory cytokines and enhancing antioxidative capability in the liver, and may become a promising adjuvant therapeutic strategy for treating liver injury induced by acute renal injury.
Le texte complet de cet article est disponible en PDF.Keywords : Renal ischemia reperfusion injury, vagus nerve stimulation, Liver injury, Organ crosstalk, Inflammation
Plan
Vol 117
Article 109062- septembre 2019 Retour au numéro
Article précédent
- Dihydroartemisinin attenuates lipopolysaccharide-induced acute kidney injury by inhibiting inflammation and oxidative stress
- Xinhui Liu, Jiandong Lu, Yijiao Liao, Siqi Liu, Yijun Chen, Riming He, Ling Men, Chunjian Lu, Zhihong Chen, Shunmin Li, Guoliang Xiong, Shudong Yang
- The oncogenic role of CB2 in the progression of non-small-cell lung cancer
- Shaohua Xu, Hanchen Ma, Yuhong Bo, Mingju Shao
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?