Iguratimod (T-614) attenuates severe acute pancreatitis by inhibiting the NLRP3 inflammasome and NF-?B pathway - 01/10/19
, Yi Miao a, b, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Highlights |
• | T-614 is a COX-2 inhibitor that exerts multiple pharmacological activities. |
• | T-614 protects mice from cerulein plus LPS induced SAP. |
• | T-614 suppresses the NF-κB signalling pathway in experimental model of SAP. |
• | T-614 inhibits the activation of NLRP3 inflammasome, and then reduce the inflammation. |
Abstract |
Severe acute pancreatitis (SAP) is an acute abdominal disease that can develop locally to the multiple organs. It is characterized by pancreatic tissue self-digestion, and the rapid release of inflammatory cytokines, which play a dominant role in local or even systemic inflammation. In this study, we investigate the protective effect of T-614 against SAP induced by cerulein plus LPS in mice. Biochemical markers associated with pancreatitis in serum such as inflammatory cytokines, amylase and lipase activities were measured. Related proteins of NLRP3 inflammasome and NF-κB signaling pathway were evaluated by western blotting. Hematoxylin-eosin staining (HE) and immunohistochemistry (IHC) were used to evaluate changes of inflammation in pancreatic tissue. T-614 significantly alleviated the elevation markers of pancreatitis and suppresses the pancreatic tissue damage, including histopathological and molecular manifestations. In conclusion, T-614 plays a protective role in experimental SAP mice model via anti-inflammatory effects.
Le texte complet de cet article est disponible en PDF.Abbreviations : T-614, LPS, COX-2, DMSO, ELISA, HE, IHC, IL, IP, MODS, NO, p, PVDF, qRT-PCR, SAP, SD, SDS-PAGE, TNF-α, NLRP3, NF-κB, AP, DMARD, NS, CAE, GAPDH, MPO, RIPA, PMSF, DAMPs, ATP, ASC
Keywords : Iguratimod, Severe acute pancreatitis, COX-2, NF-κB pathway, NLRP3
Plan
Vol 119
Article 109455- novembre 2019 Retour au numéro
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