Up-regulation of miR-498 inhibits cell proliferation, invasion and migration of hepatocellular carcinoma by targeting FOXO3 - 29/01/20
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Highlights |
• | miR-498 plays an important role in hepatocellular carcinoma. |
• | miR-498 overexpression shows anti-tumoral properties. |
• | FOXO3 is a downstream target gene of miR-498. |
Summary |
Background |
To unravel the fundamental role of miR-498 in the context of hepatocellular carcinoma cells and understands underlying potential mechanism.
Methods |
Relative viability was interrogated using MTT method and cell proliferation was determined with colony formation assay. The protein levels of cleaved Caspase-3, Bcl-2, Cyclin D, CDK4, FOXO3 and β-actin were analyzed by western blotting. Cell invasion and migration was evaluated by transwell assay and wound healing, respectively. The relative abundance of Cyclin D, CDK4, FOXO3 and miR-498 transcripts was measured using real-time PCR. The regulatory action of miR-498 on FOXO3 expression was analyzed with luciferase reporter.
Results |
Ectopic over-expression of miR-498 significantly inhibited viability and proliferation, suppressed cell migration and invasion, delayed cell cycle progression. We further identified FOXO3 as downstream target gene of miR-498, and positively modulated FOXO3 translation in miR-498-proficient cells consequently contributed to its anti-tumoral properties.
Conclusions |
Our data highlighted the tumor suppressor role of miR-498-FOXO3 signaling in hepatocellular carcinoma cells, which might hold promise for therapeutic exploitation.
Le texte complet de cet article est disponible en PDF.Keywords : miR-498, Hepatocellular carcinoma, FOXO3, Cell viability
Abbreviation : miRs
Plan
Vol 44 - N° 1
P. 29-37 - février 2020 Retour au numéro
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