Transcriptional factor Yin Yang 1 facilitates the stemness of ovarian cancer via suppressing miR-99a activity through enhancing its deacetylation level - 19/04/20
, Wei Wang a, Meng Li bBienvenue sur EM-consulte, la référence des professionnels de santé.
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Highlights |
• | A new critical role of YY1 in ovarian cancer cell stemness is proposed. |
• | The mechanisms of YY1 in ovarian cancer cell stemness are further studied. |
• | The novel mechanism provides new potential targets for YY1. |
Abstract |
The promoting effects of transcriptional factor Yin Yang 1 (YY1) have been confirmed in various tumors, however, its roles in ovarian cancer (OC) progression are still unclear. Here, Kaplan-Meier Plotter analysis was used to determine the correlation between YY1 expression and the survival of OC patients. It was found that YY1 expression was negatively correlated with the overall survival, progression-free survival and post-progression survival of OC patients. Functional experiments indicated that overexpression of YY1 facilitated the stemness of OC cells, while YY1 knockdown reduced it. MiRNAs-based RNA-sequencing analysis showed that miR-99a was the mostly upregulated miRNA in RNA extracted from OC cells with YY1 knockdown. Mechanistic studies revealed that YY1 recruited (Histone deacetylase) HDAC5 to the promoter of miR-99a, and subsequently enhanced miR-99a deacetylation level and decreased miR-99a level. Additionally, overexpression of miR-99a or knockdown of HDAC5 attenuated the promoting effects of YY1 on the stemness of OC cells. This work firstly indicated a novel YY1/miR-99a axis, which promotes the stemness of OC cells.
Le texte complet de cet article est disponible en PDF.Keywords : Yin Yang 1, miR-99a, Deacetylation, Ovarian cancer, Stemness
Plan
Vol 126
Article 110085- juin 2020 Retour au numéro
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