Overexpressed circ-RPL15 predicts poor survival and promotes the progression of gastric cancer via regulating miR-502-3p/OLFM4/STAT3 pathway - 30/05/20
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Graphical abstract |
Highlights |
• | Circ-RPL15 is upregulated in gastric cancer tissues and predicts poorer outcome of gastric cancer patients. |
• | Circ-RPL15 promotes the proliferation, migration, invasion and inhibits the apoptosis of gastric cancer cells. |
• | Circ-RPL15 functions as a competitive endogenous RNA by sponging miR-502-3p. |
• | Circ-RPL15/miR-502-3p axis regulates gastric cancer progression via modulating OLFM4/STAT4 pathway. |
Abstract |
Background |
Increasing studies have revealed that circular RNAs (circRNAs) contribute to gastric cancer (GC) progression. The circular RNA ribosomal protein L15 (circ-RPL15) is involved in chronic lymphocytic leukemia. However, its expression and functions in GC remain elusive.
Methods |
The expression of circ-RPL15 in human GC tissues and adjacent normal tissues, human gastric cancer cell lines (MGC-803, BGC-823, MGN-28, SGC-7901, AGS) and normal gastric mucosal epithelial cell line (GES-1) were detected by RT-PCR. The relationship between circ-RPL15 level and clinical-pathological indicators were also analyzed. Gain- of function experiments of circ-RPL15 and miR-502-3p were conducted to verify their roles in mediating GC cell proliferation, apoptosis and metastasis. Also, the downstream mechanisms of circ-RPL15 were predicted by bioinformatics analysis, and the interactions between circ-RPL15 and miR-502-3p, miR-502-3p and OLFM4 were verified by dual luciferase reporter gene assay and RNA FISH.
Results |
circ-RPL15 was upregulated in GC tissues and cell lines, and the overexpressed circ-RPL15 was correlated with poorer survival of GC patients. Functionally, circ-RPL15 upregulation distinctly promoted the proliferation, migration and invasion of GC cells and inhibited apoptosis. Mechanistically, circ-RPL15 functioned as a competitive endogenous RNA via sponging miR-502-3p and activated OLFM4/STAT3 pathway.
Conclusion |
circ-RPL15 promotes GC progression and predicts poor prognosis of GC patients, and regulates the malignant phenotypes of GC cells by mediating the miR-502-3p/OLFM4/STAT3 axis.
Le texte complet de cet article est disponible en PDF.Keywords : Gastric cancer, circ_RPL15, miR-502-3p, OLFM3, STAT3
Plan
Vol 127
Article 110219- juillet 2020 Retour au numéro
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