Anti-ischemia/reperfusion injury effects of notoginsenoside R1 on small molecule metabolism in rat brain after ischemic stroke as visualized by MALDI–MS imaging - 28/08/20
, Lei Wang a, b, c, d , Fang Tian e , Xin Zhao e , Xiao-Ping Pu e , Gui-Bo Sun a, b, c, ⁎ , Xiao-Bo Sun a, b, c, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Graphical abstract |
NG-R1 is a promising compound for brain protection from ischemic/reperfusion injury, possibly through the regulation of brain small molecule metabolism. NG-R1 regulated ATP metabolism, the tricarboxylic acid (TCA) cycle, the malate-aspartate shuttle, antioxidant activity, and the homeostasis of iron and phospholipids in the striatum and hippocampus of middle cerebral artery occlusion/reperfusion (MCAO/R) rats.
Highlights |
• | A new mechanism of NG-R1 in the treatment of ischemic stroke was proposed. |
• | The mechanism relies on the improvement of small molecule metabolism. |
• | A new method for the detection of metabolic small molecules was proposed. |
Abstract |
Ischemic stroke is a syndrome of severe neurological responses that cause neuronal death, damage to the neurovascular unit and inflammation. Notoginsenoside R1 (NG-R1) is a neuroprotective drug that is commonly used to treat neurodegenerative and cerebrovascular diseases. However, its potential mechanisms on the regulation of small molecule metabolism in ischemic stroke are largely unknown. The aim of this study was to explore the potential mechanisms of NG-R1 on the regulation of small molecule metabolism after ischemic stroke. Here, we found that NG-R1 reduced infarct size and improved neurological deficits by ameliorating neuronal damage and inhibiting glial activation in MCAO/R rats. Furthermore, using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we clarified that NG-R1 regulated ATP metabolism, the tricarboxylic acid (TCA) cycle, the malate-aspartate shuttle, antioxidant activity, and the homeostasis of iron and phospholipids in the striatum and hippocampus of middle cerebral artery occlusion/reperfusion (MCAO/R) rats. In general, NG-R1 is a promising compound for brain protection from ischemic/reperfusion injury, possibly through the regulation of brain small molecule metabolism.
Le texte complet de cet article est disponible en PDF.Abbreviation : MCAO/R, NG-R1, MALDI-MSI, TCA, TTC, 1,5-DAN
Keywords : Ischemic stroke, NG-R1, MALDI-MSI, Small molecule metabolism
Plan
Vol 129
Article 110470- septembre 2020 Retour au numéro
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