Validated spectrophotometric determination of maduramicin ammonium using three charge transfer complexation reactions - 30/08/20
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Highlights |
• | To the best of our knowledge, this is the first report describing the spectrophotometric determination of maduramicin since no direct spectrophotometric or colorimetric reports for this drug are available in the literature. |
• | The spectrophotometric determination of maduramicin ammonium represents an analytical challenge since it is a weak UV-absorbing compound lacking a strong chromophore. |
• | The three proposed methods are simple, rapid, cost-effective and involve single step reactions that do not require extraction or heating. |
• | The described methods can be recommended for routine and quality control analysis of maduramicin in bulk form and in powder dosage form. |
Summary |
Direct spectrophotometric determination of Maduramicin ammonium (MAD) represents an analytical challenge since it is a weak UV-absorbing and lacking a strong chromophore. This work represents the first spectrophotometric determination of MAD as no direct spectrophotometric or colorimetric determination methods for MAD are available in the literature. The present study illustrates the development of three simple, rapid and inexpensive colorimetric methods for the routine quality control analysis of MAD based on the formation of colored charge transfer complexes with three electron acceptors namely p-chloranilic acid (p-CA), 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) and picric acid (PA). The color products of MAD with p-CA, DDQ and PA were measured at 519, 588 and 405nm respectively. The proposed methods were validated in terms of linearity, ranges, precision, accuracy, robustness and limits of detection and quantification. MAD was effectively determined over concentration ranges of 100–1000, 25–250 and 30–150μg/mL using p-CA, DDQ and PA, respectively with good linearity as shown by the values of correlation coefficients not less than 0.9991. The developed methods were successfully implemented in the assay of MAD powder pharmaceutical formulation for veterinary use.
Le texte complet de cet article est disponible en PDF.Keywords : Maduramicin ammonium, Spectrophotometric determination, Charge transfer complex, P-Chloranilic acid, DDQ, Picric acid
Plan
Vol 78 - N° 5
P. 388-397 - septembre 2020 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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