Active vitamin D impedes the progression of non-alcoholic fatty liver disease by inhibiting cell senescence in a rat model - 03/09/20
, Qi Long, Fei Chen, Ting Zhang, Wenqiao WangBienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 11 |
| Iconographies | 3 |
| Vidéos | 0 |
| Autres | 0 |
Highlights |
• | Active VD impedes the progression of NAFLD. |
• | Active VD with different concentrations has varying efficiency on NAFLD. |
• | Active VD reduces oxidative stress. |
• | Active VD inhibits p53-p21 signaling pathway. |
• | Active VD suppresses cell senescence. |
Summary |
Objective |
Non-alcoholic fatty liver disease (NAFLD) refers to an accumulation of excess fat in liver due to causes other than alcohol use. The relationship between vitamin D (VD) and NAFLD has been previously studied. Therefore, we aimed to explore the mechanism involved active VD regulating the progression of NAFLD by inhibiting cell senescence and to provide a potential approach for further nutritional treatment of NAFLD.
Methods |
Following the induction with high-fat diet and intraperitoneal injection of corn oil, the successfully established NAFLD rat models were treated with 1,25(OH)2D3 at 1μg/kg, 5μg/kg or 10μg/kg. Meanwhile, the levels of factors related to oxidative stress, cell senescence, the p53-p21 signaling pathway and inflammation in liver were determined. Then, cell senescence was also measured by using senescence-associated β-galactosidase (SAβ-gal) staining.
Results |
It was also found that active VD increased the concentration of VD in serum and VDR in liver of NAFLD rats, and alleviated hepatic fibrosis. Besides, treatment of 1,25(OH)2D3 at 1μg/kg, 5μg/kg or 10μg/kg reduced oxidative stress and inflammation, inhibited the p53-p21 signaling pathway and consequent cell senescence. Furthermore, treatment of 1,25(OH)2D3 at a dosage of 5μg/kg made the most impact on these factors.
Conclusion |
Collectively, the evidences from this study demonstrated that active VD could alleviate the development of NAFLD through blocking the p53-p21 signaling pathway, which provided a novel nutritional therapeutic insight for NAFLD.
Le texte complet de cet article est disponible en PDF.Keywords : Non-alcoholic fatty liver disease, Active vitamin D, Oxidative stress, Cell senescence, P53-p21 signaling pathway, Vitamin D receptor
Abbreviations : NAFLD, VD, ALT, TBARS, MDA, TAOC, TNF
Plan
Vol 44 - N° 4
P. 513-523 - septembre 2020 Retour au numéro
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