Mediterranean mountain ecosystems have been attractive to human societies due to their valuable resources, but are also susceptible to environmental and climate changes. The Rhodope Mountain Range hosts one of the least disturbed natural forests of Europe and is a conservation priority area in the Southern Balkans. Located in the borderlands of the plain of Macedonia, the forest ecosystem development of Rhodope Mountains was shaped not only by Late Holocene climatic variability, but also by changes in human activities since Byzantine times. Palynological and microscopic charcoal analysis of the Livaditis ombrotrophic bog record offers unique insights into vegetation and landscape evolution under the influence of human land-use practices in the south Rhodope area during the last c.a. 1100 years. The findings show a forested landscape, with well-developed Pinus and Abies forests of in the montane zone and mixed deciduous oak forests below that flourished in the area until 900 AD. The expansion of human activity in mountainous areas during the period of Byzantine economic growth (ca. 1000 AD) is evidenced by forest clearance through fire, affecting mainly the Abies populations. The Livaditis record bears evidence about both, arboriculture and cereal cultivation as well as animal husbandry during the first period of human activities in the uplands, while a shift towards pastoralism is most likely associated with the establishment of the Vlach population in the region (ca. 1200 AD). Subsequently, a short-lived expansion of the Pinus percentages could be attributed to the afforestation of abandoned pasture land during the Little Ice Age. Finally, a further intensification of pastoralism is most likely concurrent with the population expansion documented during the Ottoman period (after 1500 AD). The Livaditis pollen record shows significant vegetation shifts in the upland area of southern Rhodope Mountains that could be associated with changes in climate, population mobility and density, as well as evolving land-use practices.