Exogenous growth differentiation factor 15 (GDF-15) exerts direct cardioprotective properties towards myocardial ischemia reperfusion injury - 25/09/20
Résumé |
Introduction |
Growth differentiation factor 15 (GDF15) is a stress-responsive cytokine, which can be produced under certain pathological situations, mainly related to inflammatory stress, aging and disease. While clinical data suggest that GDF15 is a powerful risk factor in several high cardiovascular risks situations such as myocardial infarction and coronary artery disease, experimental studies rather consider this cytokine as a cardioprotective molecule.
Objective |
We therefore aimed to clarify the direct cardiac effects of GDF15 during ischemia-reperfusion (I-R) injury, at concentrations found in patients with high cardiovascular risk, such as these of patients presenting with acute myocardial infarction or stroke.
Method |
Wistar male rats (250–400g) were anesthetized with isoflurane before their hearts were isolated and perfused in a Langendorff model. Hearts were perfused either with saline (control groups) or 2000ng/L of GDF15 upstream the coronary bed (GDF15 groups) for 10minutes, then subjected to either 20 or 30minutes of global normothermic total ischemia, followed by 2h of reperfusion.
Results |
GDF15 induced a better recovery of most contractile parameters after 30minutes of ischemia (higher left ventricular developed pressure, better heart rate recovery, better recovery in left ventricular contractility and relaxation, P<0.05 for all parameters). Moreover, after an irreversible 30-minute duration of ischemia, GDF15 decreased infarct size (45.6±3.8% vs. 60.8±3.8%, P<0.05).
Conclusion |
These preliminary results demonstrate for the first time in an ex vivo isolated heart model, that exogenous preischemic short-term administration of 2000ng/L exerts cardioprotective properties towards ischemia reperfusion injury, improving recovery of functional parameters and decreasing cardiac cell death.
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Vol 12 - N° 2-4
P. 228 - octobre 2020 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.