The intranasal administration of Carthamus tinctorius L. extract/phospholipid complex in the treatment of cerebral infarction via the TNF-?/MAPK pathway - 27/10/20
, Junbo Zou a, b, Xiaofei Zhang a, b, Mei Wang a, b, Dongyan Guo a, b, Guiyuan Lv c, Jie Su c, Ting Wang cBienvenue sur EM-consulte, la référence des professionnels de santé.
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Graphical abstract |
In this study, we used database retrieval to select the main chemical compositions and disease targets of traditional Chinese medicine and constructed the composition-target-disease network. GO enrichment analysis and KEGG enrichment pathway analysis were deployed via the R software package. Our aim was to explore potential pathways underlying the action of Safflower in the treatment of CI, to establish a model MCAO, and to study the mechanisms associated with the action of Safflower at the molecular level.
Highlights |
• | We identified 52 effective targets for Safflower in the treatment of CI. |
• | We confirmed that the mechanism responsible for the action of Safflower in the treatment of CI was the CASP3 mediation of the TNF-α / MAPK pathway. |
• | Our analyses identified several targets, including TNF-α, CASP3, MAP3K1, JNK, AP1, IL-1 β, and IL-6. |
• | Safflower had a protective effect on CI by improving blood circulation, inhibiting apoptosis and protecting the brain cells. |
• | Our results provide preliminary evidence for the pharmacological mechanism of Safflower in the treatment of CI. |
Abstract |
Carthamus tinctorius L.(Safflower), a herbal formula from Traditional Chinese Medicine (TCM), has been widely used for the treatment of cardio-cerebrovascular diseases, particularly cerebral infarction (CI) or cerebral ischemia-reperfusion injury. However, we know very little about the specific mechanisms associated with the therapeutic effect of Safflower on CI. In this study, we used a network pharmacology-based approach, together with rat model of CI, to gain more insight into of such mechanisms. Our analysis showed that Safflower contains 52 active compounds that target 247 genes, which were also cross-referenced with 299 genes associated with CI. Consequently, we identified 52 target genes in Safflower that were associated with CI. These 52 target genes were analyzed by gene ontology (GO) enrichment analysis, leading to the identification of 1491 biological process items, 90 molecular function items and 19 cell assembly items. Eighty-nine pathways were generated by KEGG enrichment (P < 0.05). Next, we investigated the effect of the extract of safflower (ES) and Safflower extract phospholipid complex (ESPC), delivered via the nasal route, on an animal model of the middle cerebral artery occlusion (MCAO). Our data confirmed that Safflower was able to treat CI by the regulating the TNF-α/MAPK pathway via CASP3. The therapeutic effect of ES and ESPC on CI acts by improving the circulation of blood in the central nervous system, reducing the inflammatory reaction, inhibiting apoptosis, and by protecting brain nerve cells from injury.
Le texte complet de cet article est disponible en PDF.Abbreviations : Safflower, CI, GO, MCAO, ES, ESPC
Keywords : Carthamus tinctorius L., Cerebral infarction, TNF-α/MAPK pathway, Safflower extract phospholipid complex, Network pharmacology
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Vol 130
Article 110563- octobre 2020 Retour au numéro
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