Induced pluripotent stem cell-derived neural progenitor cell transplantation promotes regeneration and functional recovery after post-traumatic stress disorder in rats - 19/12/20
, Junfeng Zhang a, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Graphical abstract |
Highlights |
• | Post-traumatic stress disorder (PTSD) is a mental disorder characterized by hippocampal neuron loss and cognitive dysfunction. |
• | Induced pluripotent stem cell (iPSCs) differentiated into neural progenitor cells (iPSC-NPCs) in vitro. |
• | IPSC-NPCs transplantated into PTSD rats, they can promote regeneration and motor function recovery in PTSD model. |
• | The open field and fear condition test were used to assess the recovery of the PTSD. |
Abstract |
Post-traumatic stress disorder (PTSD) is a mental disorder characterized by hippocampal neuron loss and cognitive dysfunction. The aim of the present study was to investigate the potential functional outcomes of transplantation of induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs) for treating PTSD. Human induced pluripotent stem cell (iPSCs), differentiated into neural progenitor cells (NPCs) in vitro, were transplanted into the brain of rat. Following iPSC-NPCs transplantation, cognitive function was determined. The open field test and fear condition test indicated that long-term iPSC-NPCs transplantation ameliorated cognitive dysfunction and reduced freezing time in PTSD rats. Following testing, the brain of rat was analyzed using immunocytochemistry and immunofluorescence. The results revealed that iPSC-NPCs differentiated into neurons replacing the loss of hippocampus neurons, and iPSC-NPCs transplantation showed higher expression of glial fibrillary acidic protein (GFAP) and increased number of NeuN compared with the control group. Moreover, western blot analysis suggested enhanced expression of brain-derived neurotrophic factor (BDNF) in hippocampus tissue of iPSC-NPCs transplanted rats in comparison to the PBS group. Collectively, these findings showed that iPSC-NPCs could promote regeneration and motor function recovery in PTSD model.
Le texte complet de cet article est disponible en PDF.Abbreviations : PTSD, GFAP, BDNF, iPSCs, NPCs
Keywords : Post-traumatic stress disorder, Induced pluripotent stem cell, Neural progenitor cells, GFAP, BDNF
Plan
Vol 133
Article 110981- janvier 2021 Retour au numéro
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