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Comparative evaluation of DTT treated ABO isoagglutinin titers performed by two methods with solid phase red cell adhesion (SPRCA) titers - 13/01/21

Doi : 10.1016/j.tracli.2021.01.002 
Prashant Pandey a, , Divya Setya a, Shweta Ranjan a, Mukesh Kumar Singh b
a Department of Transfusion Medicine, Histocompatibility and Molecular Biology, Jaypee hospital, Sector-128, Noida-201304 
b Senior Scientific Officer, Department of Transfusion Medicine, Jaypee Hospital, Sector 128, Noida 

Corresponding author. Additional Director, Department of Transfusion Medicine, Histocompatibility and Molecular Biology, Jaypee hospital, Sector-128, Noida-201304, Phone No:+91 9910449847, Fax: +0120-4582899. 9910443847.Additional Director, Department of Transfusion Medicine, Histocompatibility and Molecular Biology, Jaypee hospital, Sector-128, Noida-201304, Phone No:+91 9910449847, Fax: +0120-4582899. 9910443847
Sous presse. Manuscrit accepté. Disponible en ligne depuis le Wednesday 13 January 2021

Abstract

Background and aims

Measurement of actual concentration of IgG requires methods like treatment of serum with dithiothreitol (DTT). This study was aimed at comparing of DTT treated ABO titers performed by conventional test tube technique (CTT) and column agglutination technique (CAT) with HA/SPRCA.

Materials and methods

This was a prospective, observational study conducted from October 2019 to March 2020. All consecutive A, B and O group donors who gave consent for participation were included. All samples were tested by CTT and CAT before and after DTT treatment (pCTT, pCAT) and with HA/SPRCA.

Results

A total of 300 donors were included; 100 each from A, B and O blood group donors. Group O titers were higher than group A/ B titers. Group O titers were highest when performed by pCAT, followed by pCTT and lowest by HA/ SPRCA. Group A/ B titers were highest when performed by HA/ SPRCA, followed by pCAT and pCTT for anti-A and highest when performed by pCAT, followed by HA/ SPRCA and lowest by pCTT for anti-B.

Conclusion

Results obtained by pCAT were closer to results obtained by pCTT, whereas those obtained by HA/ SPRCA were variable. SPRCA offers the advantage of automation, no inter-observer variartion and less time consumption because IgM interference is not observed with SPRCA, thus providing an alternative to pCTT. However, these methods cannot be used interchangeably and to discern the most suitable method, a clinical impact of these results needs to be studied.

Le texte complet de cet article est disponible en PDF.

Keywords : Conventional tube technique, Column agglutination technology, ABO, Titration, DTT, SPRCA



© 2021  Publié par Elsevier Masson SAS.
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