Hepatoprotective effects of extracts, fractions and compounds from the stem bark of Pentaclethra macrophylla Benth: Evidence from in vitro and in vivo studies - 19/02/21
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Graphical abstract |
Highlights |
• | Pentaclethra macrophylla extracts and fractions induced concentration-dependent inhibition of lipid peroxidation greater than that of silymarin. |
• | The purification of the EtOAc fraction yielded 7 compounds among which 11-O-galloylbergenin was the most effective. |
• | In vivo, the EtOAc fraction significantly reduced the serum level of ALAT, ASAT and TNF-α in d-galactosamine/LPS-induced hepatitis in mice. |
• | The EtOAC fraction of P. macrophylla protected the liver against structural damages, but did not boost the endogenous antioxidant parameters. |
Abstract |
Aim |
To identify the bioactive hepatoprotective components of the ethanol extract of Pentaclethra macrophylla stem bark using in vitro and in vivo approaches.
Methods |
The bioguided-fractionation of the ethanol extract was based on the substances’ capacity to prevent in vitro, the lipid peroxidation of hepatocytes’ membranes induced by hydrogen peroxide. For the in vivo hepatoprotective test, mice were treated orally with the ethyl acetate (EtOAc) fraction of the ethanol extract at doses of 50 and 75 mg/kg/day for one week and subjected to d-galactosamine/lipopolysaccharide (GaIN/LPS)-induced hepatotoxicity. Blood samples were collected for alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), TNF-α and IL-1β assays. The liver was harvested for histological and biochemical (proteins, glutathione (GSH), catalase and superoxide dismutase (SOD)) analysis.
Results |
The ethanol extract and fractions induced concentration-dependent inhibition of lipid peroxidation (IC50: 3.21–48.90 μg/mL) greater than that of silymarin (IC50: 117.4 μg/mL). The purification of the sub-fractions of EtOAc fraction yielded: (7R)-7-hydroxyhexacosanoic acid (1), (7R)-1-(7-hydroxyhexacosanoyl) glycerol (2), bergenin (3), 11-O-galloylbergenin (4), 2-hydroxymethyl-5-(2-hydroxypropan-2-yl)phenol (5), β-sitosterol 3-O-β-d-glucopyranosyl (6) and β-sitosterol (7)), among which 11-O-galloylbergenin (IC50:1.8 μg/mL) was the most effective. The EtOAc fraction significantly reduced the serum level of ALAT, ASAT and TNF-α in vivo. This EtOAc fraction increased the liver protein content and protected the liver against structural damages, but did not boost the endogenous antioxidant parameters.
Conclusion |
The stem bark of Pentaclethra macrophylla possesses hepatoprotective effects that may result from its capacity to inhibit lipid peroxidation and could be attributed to its active components 3, 4 and 2.
Le texte complet de cet article est disponible en PDF.Abbreviations : ALAT, ASAT, 13C-NMR, EI, EtOAc, GaIN/LPS, GSH, 1H-NMR, 1H-1H-COSY, HMBC, HSQC, MeOH, n-BuOH, SOD
Keywords : Pentaclethra macrophylla, Isolated compounds, Hepatoprotection, Structure-activity relationships, Lipid peroxidation, GaIN/LPS
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Vol 136
Article 111242- avril 2021 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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