A novel amphibian-derived peptide alleviated ultraviolet B-induced photodamage in mice - 19/02/21
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Highlights |
• | In this research, we identify a new peptide (OM-GL15) from the skin of the Odorrana margaretae. |
• | The experimental results show that OM-GL15 can inhibit the expression of p53 protein. |
• | OM-GL15 inhibit the mitochondrial apoptosis pathway mediated by caspase-9 and caspase-3. |
• | OM-GL15 has potential application value in the development of anti-UVB skin photodamage drugs. |
Abstract |
Although the application potential of amphibian skin-derived active peptides in alleviating ultraviolet B (UVB)-induced damage has attracted increasing attention, research remains in its infancy. In this study, a new peptide (OM-GL15, GLLSGHYGRASPVAC) was identified from the skin of the green odorous frog (Odorrana margaretae). Results showed that OM-GL15 scavenged free radicals (2,2′-diazo-bis-3-ethylbenzothiazoline-6-sulfonic acid and 1,1-diphenyl-2-trinitrophenylhydrazine) and reduced Fe3+ to Fe2+. Moreover, topical administration of OM-GL15 significantly alleviated UVB-induced skin photodamage in mice. Exploration of the underlying mechanisms further showed that OM-GL15 exerted antioxidant potency. Specifically, the peptide reduced the levels of lipid peroxidation and malondialdehyde and protected epidermal cells from UVB-induced apoptosis by inhibiting DNA damage via down-regulation of p53, caspase-3, caspase-9, and Bax and up-regulation of Bcl-2. Our results highlight the potential application of amphibian skin-derived peptides in protection against UVB-induced photodamage and provide a novel peptide candidate for the development of anti-photodamage agents.
Le texte complet de cet article est disponible en PDF.Keywords : Skin, Acute photodamage, Antioxidant peptide, DNA damage, Apoptosis
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Article 111258- avril 2021 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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