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Engineering of axially vascularized bone tissue using natural coral scaffold and osteogenic bone marrow mesenchymal stem cell sheets - 23/02/21

Doi : 10.1016/j.jormas.2021.01.013 
Yanming Weng a, 1, Zhifa Wang b, c, 1, Jianwei Sun d, Leng Han e, Xiao Li c, Buling Wu b, , Qingshan Dong a, , Yanpu liu f,
a Department of Stomatology, General Hospital of Central Theater of PLA, Wuhan 430070, China 
b School of Stomatology, Southern Medical University, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China 
c Department of Stomatology, General Hospital of Southern Theater of PLA, Guangzhou 510010, China 
d School of Stomatology, Guangzhou Special Service Recuperation Center of PLA Rocket Force, Guangzhou 510010, China 
e Department of Pathology, General Hospital of Southern Theater of PLA, Guangzhou 510010, China 
f State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi’an 710032, China 

Corresponding author at: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi’an 710032, China.State Key Laboratory of Military StomatologyDepartment of Oral and Maxillofacial SurgerySchool of Stomatologythe Fourth Military Medical University145 West Changle RoadXi’an710032China
Sous presse. Épreuves corrigées par l'auteur. Disponible en ligne depuis le Tuesday 23 February 2021

Abstract

Introduction

Blood supply remains one of the obstacles to large bone tissue engineering. This study aimed to generate vascularized bone tissue by inducing axial vascularization into a construct combining natural coral scaffold and a bone marrow mesenchymal stem cells (BMSCs) sheet.

Material and methods

Isolated BMSCs were cultured to form an osteogenic cell sheet using a continuous culture method. Natural coral scaffolds were prepared into customized shape with a cylinder of 20 mm length, 8 mm in outer diameter and 5 mm in inner diameter. Then, the freed superficial inferior epigastric vessel of rabbits was first wrapped with a cell sheet, and then inserted into the central passage of the scaffold, after being wrapped with another cell sheet, the complexes were implanted subcutaneously into a rabbit groin area. In contrast, the sheet-scaffold construct that implanted into groin subcutaneous area of the other side of the same rabbit with the distal end of the blood vessel was ligated, which was considered as control. New bone and vascularization formation were evaluated at 12 weeks postoperatively.

Results

The volume of new bone formation and amount of capillary infiltration in the vascular circulation group were significantly greater than that in the vascular ligation group, which suggested that insertion of axial vessels could significantly promote angiogenesis and osteogenesis of the tissue-engineered bone.

Conclusions

These findings indicate that inserting an arteriovenous bundle into the constructs of mesenchymal stem cell sheet and coral has great potential for clinical applications to repair large bone defects.

Le texte complet de cet article est disponible en PDF.

Abbreviations : BMSCs, BTE, ECM, DMEM/F12, FBS, H&E, SEM, MPTC, SD, BMP-2, COL I, OCN, OPN, CT

Keywords : Bone marrow mesenchymal stem cells, Cell sheet, Vascularization, Coral scaffold, Bone tissue engineering


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