Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells - 16/04/21
, Wisam Nabeel Ibrahim f, g, ⁎ , Hussah Abdullah Alshwyeh h, Areej A Alosaimi h, Sahar Khamees Aldosary h, Eltayeb E.M. Eid i, ⁎ , Rozita Rosli a, Y.H. Taufiq-Yap j, k, Nagi A. Al-Haj a, l, Mothanna Sadiq Al-Qubaisi aBienvenue sur EM-consulte, la référence des professionnels de santé.
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Abstract |
The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinum-doped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anti-cancer compound.
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Highlights |
• | Pt/MgO nanoparticles at 12.5 µg/mL selectively killed lung and colon cancer cells while leaving normal cells unaffected. |
• | Pt/MgO nanoparticles induces oxidative stress-mediated apoptosis. |
• | The apoptotic effects of Pt/MgO nanoparticles on cancer cells were via both the intrinsic and the extrinsic pathways. |
Keywords : Pt/MgO nanoparticles, Cytotoxicity, Oxidative stress, Lipid peroxidation, Apoptosis, Lung cancer, Colonic cancer
Plan
| ☆ | The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. |
Vol 138
Article 111483- juin 2021 Retour au numéro
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