In vivo and ex vivo longitudinal follow-up of resveratrol supplementation or restauration of a normal diet in female rat hearts submitted to high-fat-high-sucrose diet - 18/05/21
Résumé |
Introduction |
Prediabetic women are at greater risk of cardiovascular diseases than men, investigating new sex dependent strategic therapies is then essential to limit cardiovascular complications in prediabetic women.
Objective |
We aimed to evaluate the effects of resveratrol supplementation (RSV) or restauration of a standard diet on the heart of prediabetic female rats submitted to high-fat-high-sucrose diet (HFS).
Methods |
Wistar female rats were divided in 4 groups fed for 5months with: normal or HFS diet (CTRL/HFS), HFS with RSV (1mg/kg/day) during the last 2months or HFS for 3months and 2months of standard diet (RSD). We performed a longitudinal in vivo study of cardiac function, morphology and perfusion by MRI, then rats underwent an IPGTT. Ex vivo experiments on isolated perfused hearts were realized to study cardiac function [rate pressure product (RPP), end diastolic pressure (EDP)] and energy metabolism with 31P magnetic resonance spectroscopy during an ischemia-reperfusion injury (IR). Tissues were collected for analyzes.
Results |
Five months of HFS diet-induced glucose intolerance (P<0.05), increased heart perfusion and heart/tibia length ratio (HTLR) (P<0.01 vs. CTRL). HFS also induced altered myocardial tolerance to IR, characterized by impaired RPP and EDP (P<0.001) associated with lower ATP and PCr levels during reperfusion (P<0.001, P<0.05 vs. CTRL). RSV had no effect on glucose tolerance and heart perfusion (P<0.01 vs. CTRL) but normalized HTLR to CTRL level. Interestingly, RSV diminished EDP during reperfusion (P<0.05 vs. HFS). RSD normalized glucose tolerance without effect on heart perfusion and HTLR (P<0.01 and P<0.05 vs. CTRL). RSD improved tolerance to IR with increased RPP (P<0.05 vs. HFS), ATP and PCr levels during reperfusion (P<0.01, P<0.001 vs. HFS).
Conclusion |
The two approaches have distinct effects on cardiac function and energy metabolism of prediabetic female rats. Further studies are on the go to explore the mechanisms involved.
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Vol 13 - N° 2
P. 198 - mai 2021 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.