The pro-apoptotic properties of a phytonutrient rich infusion of A. cherimola leaf extract on AML cells - 17/06/21
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Abstract |
Annonaceae family has broad uses in herbal medicine for treatment of several diseases, whether through seeds’ or leaves’ extracts. The present study investigates the antiproliferative and antitumor activity of Annona cherimola aqueous leaf (AAL) extract/infusion in acute myeloid leukemia (AML) cell lines in vitro. High-resolution LC-MS was first used to analyze the composition of the aqueous extract. Cell proliferation assay, Annexin V staining, cell cycle analysis, dual Annexin V/PI staining, cell death quantification by ELISA, ROS level detection and Western Blotting were then performed to elucidate the therapeutic effects of AAL extract. The results obtained revealed a potent antioxidant activity of AAL extract. Moreover, the extract exhibited dose- and time-dependent antiproliferative effects on AML cell lines by decreasing cell viability with an IC50 of 5.03% (v/v) at 24 h of treatment of KG-1 cells. This decrease in viability was accompanied with a significant increase in apoptotic cell death with cell cycle arrest and flipping of the phosphatidylserine from the inner to the outer leaflet of the cell membrane. The respective overexpression and downregulation of proapoptotic proteins like cleaved caspase-8, cleaved PARP-1 and Bax and antiapoptotic proteins like Bcl-2 further validated the apoptotic pathway induced by AAL on AML cells. Finally, LC-MS revealed the presence of several compounds like fatty acids, terpenes, phenolics, cinnamic acids and flavonoids that could contribute to the antioxidant and anti-cancer effects of this herbal infusion. In addition to the generally known nutritional effects of the Annona cherimola fruit and leaves, the presented data validates the antioxidant and anti-cancerous effects of the leaf infusion on AML cell lines, proposing its potential therapeutic use against acute myeloid leukemia with future in vivo and clinical trials.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Annona cherimola leaf extract inhibits the proliferation of AML cell lines. |
• | A. cherimola leaf extract induces apoptosis in AML cells. |
• | A. cherimola leaf extract scavenges Reactive Oxygen Species in AML cells. |
• | A. cherimola tea has compounds contributing to its protective effects. |
Abbreviation : AAL, AML, DMEM, FBS, IC 50, LC-MS, MNCs, PI, ROS, RPMI
Keywords : Annona cherimola tea infusion, Anti-oxidant, Apoptosis, Phytochemicals, Acute Myeloid Leukemia
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Vol 140
Article 111592- août 2021 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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