Glavonoid-rich oil supplementation reduces stearoyl-coenzyme A desaturase 1 expression and improves systemic metabolism in diabetic, obese KK-Ay mice - 17/06/21
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Abstract |
Aims |
Glavonoid-rich oil (GRO) derived from ethanol extraction of licorice (Glycyrrhiza glabra Linne) root has been reported to have beneficial effects on health. In this study, we aimed to determine the effect of long-term administration of GRO on metabolic disorders and to elucidate the molecular mechanism.
Main methods |
Female obese, type 2 diabetic KK-Ay mice were fed diets supplemented with 0.3% or 0.8% GRO (w/w) for 4–12 weeks. Mice were euthanized and autopsied at 20 weeks old. The effects of GRO on lipid and glucose metabolism were evaluated by measuring physiological and biochemical markers using mRNA sequencing, quantitative reverse-transcription PCR, and western blot analyses.
Key findings |
Compared to mice fed the control diet, GRO-supplemented mice had reduced body and white adipose tissue weights, serum levels of triglycerides and cholesterol, and improved glucose tolerance, while food intake was not affected. We found remarkable reductions in the gene expression levels of stearoyl-coenzyme A desaturase 1 (Scd1) and pyruvate dehydrogenase kinase isoenzyme 4 (Pdk4) in the liver, in addition to decreased expression of fatty acid synthase (Fasn) in inguinal white adipose tissue (iWAT). These results suggest that GRO supplementation improves lipid profiles via reduced de novo lipogenesis in the liver and white adipose tissue. Glucose metabolism may also be improved by increased glycolysis in the liver.
Significance |
Our analysis of long-term supplementation of GRO in obese and diabetic mice should provide novel insight into preventing insulin resistance and metabolic syndromes.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Glavonoid-rich oil (GRO) supplementation reduces amount of white adipose tissue. |
• | GRO improves glucose tolerance in obese KK-Ay mice. |
• | GRO regulates fatty acid synthase (FAS) expression in iWAT. |
• | GRO regulates glucose switch PDK4 and fatty acid enzyme SCD1 expression in liver. |
• | Therapeutic use of GRO could prevent insulin resistance and metabolic syndrome. |
Keywords : Licorice flavonoid, Glabridin, Stearoyl-coenzyme A desaturase 1, KK-Ay mice, Lipid metabolism
Plan
Vol 140
Article 111714- août 2021 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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