Oligonucleotide aptamers: Recent advances in their screening, molecular conformation and therapeutic applications - 09/10/21
, Zhongcheng Liu a, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Abstract |
Aptamers are single stranded oligonucleotides with specific recognition and binding ability to target molecules, which can be obtained by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Aptamers have the advantages of low molecular weight, low immunogenicity, easy modification and high stability. They play promising role in promoting food safety, monitoring the environment and basic research, especially in clinical diagnosis and therapeutic drugs. To date, great achievements regarding the selection, modifications and application of aptamers have been made. However, since it is still a challenge to obtain aptamers with high affinity in a more effective way, few aptamer-based products have already successfully entered into clinical use. This review aims to provide a thorough overview of the latest advances in this rapidly developing field, focusing on aptamer screening methods for different targets, the structure of the interaction between aptamers and target substances, and the challenges and potential of current therapeutic aptamers.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Various screening methods were designed for different targets in order to improve the screening efficiency. |
• | The mechanism of aptamer focuses on spatial structure, binding domains and key binding sites with target substances. |
• | RNA and DNA aptamers have undergone clinical trials for the treatment of various diseases and are still under active development. |
Abbreviations : AFM, ACS, AMD, ApDC, bFGF, BSA, CE, CLL, CpDC, DCF, DES, ECEEM, FACS-SELEX, FCE-SELEX, GO, HAS, HDLBP, HIV, IFCE, IFCE, ITC, KGF, LIGS-SELEX, LPSs, LRPPRC, MB, MMC, MRGO, MS, MST, Nampt, NECEEM, NGS, NMR, PDGF, PectI-SELEX, PMM-SELEX, qPCR, REG1 system, scCE-SELEX, SCLC, SELEX, SPR, ssDNA, TTP, VEGF, vWF
Keywords : Aptamer, SELEX, Screening method, Nucleic acid drug, Clinical trials
Plan
Vol 143
Article 112232- novembre 2021 Retour au numéro
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