Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota - 13/11/21
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Abstract |
In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis. RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages. In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis. The diversity of the fecal microbiota was significantly increased in the group treated with RSE. In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia. Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice. For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects. Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | RSE decreased the production of cytokines in LPS-stimulated macrophage. |
• | RSE decreased colitis symptoms in DSS-colitis mice. |
• | RSE show recovery effect of intestinal dysbiosis in DSS-colitis mice. |
• | 1,2-O-disinapoyl glucoside was found in RSE that show anti-inflammatory effect. |
Keywords : Colitis, Inflammatory cytokines, Microbiota, Radish sprout (RS), Dextran sulfate sodium (DSS)
Plan
Vol 144
Article 112365- décembre 2021 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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