Can the essential oil of rosemary (Rosmarinus officinalis Linn.) protect rats infected with itraconazole-resistant Sporothrix brasiliensis from fungal spread? - 02/12/21
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Highlights |
• | Itraconazole-resistant Sporothrix brasiliensis was inoculated into the footpad of rats. |
• | Skin lesions were recovered after 30 days of daily oral treatment with rosemary oil. |
• | Rosemary oil delayed the fungal spreading, protecting the liver and spleen. |
• | The antifungal action occurred at the level of complexation with fungal ergosterol. |
• | The major compounds were 1,8-cineole/eucalyptol, camphor, and α-pinene. |
Abstract |
Introduction |
Itraconazole is the first-choice option to treat human and animal sporotrichosis. However, the emergence of itraconazole-resistant strains has encouraged research on new active antifungals. Among them, the essential oil of rosemary (Rosmarinus officinalis Linn., Lamiaceae) has shown antifungal activity in vitro.
Objective |
Assessing, for the first time, the effectiveness of rosemary essential oil in vivo in experimental cutaneous sporotrichosis, as well as its chemical composition and action mode.
Methods |
Itraconazole-resistant Sporothrix brasiliensis was inoculated in the left foot pad of 30 Wistar rats, which were randomized (n=10) for treatment with saline solution (control, CONT), itraconazole (ITRA, 10 mg/kg) and rosemary oil (ROSM, 250 mg/kg) for 30 days at an oral dose of 1 mL, daily. Clinical evolution, histopathology and fungal burden were investigated. GC-MS was used for chemical analysis; sorbitol protection and ergosterol effect were used to evaluate the action mechanism of rosemary oil.
Results |
ROSM was the only group evolving to skin lesion remission, lack of edema and exudate, and mild-to-absent yeast cells. Rosemary oil delayed fungal spreading and protected systemic organs, mainly liver and spleen. The ROSM group presented lower fungal load than that observed for the CONT and ITRA groups (p<0.05). Antifungal action took place at complexation level after ergosterol application. Most compounds were 1,8-cineole/eucalyptol (47.91%), camphor (17.92%), and α-pinene (11.52%).
Conclusions |
These findings have evidenced that rosemary oil is a promising antifungal to treat sporotrichosis, since it protects systemic organs from fungal spread.
Le texte complet de cet article est disponible en PDF.Keywords : Sporotrichosis, Itraconazole, Antifungal resistance, Natural Product, Rosmarinus officinalis L., Action mechanism
Plan
Vol 31 - N° 4
Article 101199- décembre 2021 Retour au numéro
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