Molecular identification and antifungal susceptibility of 75 clinical isolates of Trichophyton spp. from southern Brazil - 02/12/21
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 5 |
| Iconographies | 1 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Background |
Dermatophytoses affect more than 20% of the world's population and is caused by filamentous fungi, mainly of the genus Trichophyton. The species identification through microscopic direct examination and culture methods is challenging, with molecular presenting high sensitivity and specificity. Although there are several therapy options for dermatophyte infections, treatment failures and antifungal resistance are growing concerns.
Objective |
This study aimed to identify clinical isolates of Trichophyton spp. from southern Brazil using molecular methods and determine their in-vitro antifungal susceptibility.
Material and Methods |
Seventy-five isolates were identified through sequencing of the ITS region. The exposure to seven antifungals drugs was performed according to protocol M28-A2 of the Clinical and Laboratory Standards Institute (CLSI).
Results |
Sixty-one isolates (81%) were identified as T. interdigitale, which differs from the epidemiological data present in the literature. Thirteen isolates were identified as T. rubrum and one as T. tonsurans. Terbinafine was the most effective antifungal, followed by itraconazole and voriconazole, which is in accordance with the results reported in previous studies.
Conclusions |
The use of molecular methods to identify Trichophyton spp. clinical isolates and the performance of susceptibility tests are relevant to epidemiological data, identification of the emergence of antifungal resistance, and to help to translate the in-vitro antifungal susceptibility results into clinical practice.
Le texte complet de cet article est disponible en PDF.Keywords : Dermatophytoses, Trichophyton, Molecular identification, Antifungal susceptibility
Plan
Vol 31 - N° 4
Article 101201- décembre 2021 Retour au numéro
Article précédent
- Invasive fungal infections among critically ill adult COVID-19 patients: First experiences from the national centre in Hungary
- Balint Gergely Szabo, Botond Lakatos, Ilona Bobek, Edina Szabo, Janos Szlavik, István Vályi-Nagy
- HLA-DRB1 alleles as predisposing and resisting factor in women suffering from vulvovaginal candidiasis
- Tandis Razavi, Mehraban Falahati, Shahram Teimourian, Shirin Farahyar, Vahid Babaei, Niloufar Majdabadi
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?