to determine some pharmacokinetic parameters in mice after oral and IV administrations of hydroalcoholic extract of Nerium oleander.

Pharmacokinetic studies of oleandrin, a cardiotonic glycoside and main active compound of Nerium oleander were conducted in mice after administration of an IV dose (30mg/kg) and a PO dose (150mg/kg) of an hydrolcoholic extract of Nerium oleander. Oleandrin was measured by LC-MS/MS.

oleandrin was rapidly absorbed after oral administration (Cmax at 10min). The AUC0-inf (ug/L*min) values obtained after IV and PO dosing were 34,797.7 and 107,222, respectively, resulting in an oral bioavailability of 61.6%. The apparent volume of distribution Vss was 0.55L/kg and clearance ClT was 0.01kg* L/min.

Further studies are needed, to elicit the pharmacokinetic parameters of oleandrin after various routes of administration, in multiple species, multiple doses et and different preparations.