Piceatannol-3′-O-β-D-glucopyranoside attenuates colistin-induced neurotoxicity by suppressing oxidative stress via the NRF2/HO-1 pathway - 28/03/23
Abstract |
Background |
Multidrug-resistant Gram-negative bacteria are the most pressing problem in treating infectious diseases. As one of the primary drugs for multidrug-resistant Gram-negative bacteria, the neurotoxicity of colistin has become a significant challenge in clinical practice.
Purpose |
This study aimed to investigate the potential effect of piceatannol-3′-O-β-D glucopyranoside (PG) on colistin-induced neurotoxicity and the underlying mechanism.
Methods |
In vitro, nerve cell damage models were established by exposing N2a cells to 400 μM colistin for 24 h. The effects of PG on cell viability, apoptosis level, and oxidative stress level were analyzed. A western blot experiment was performed to determine the NRF2 pathway, apoptosis, and autophagy-related proteins. Mitochondrial morphology and mitochondrial membrane potential were detected after staining using laser confocal microscopy. In vivo, nerve injury mouse model was established by intracerebroventricular colistin administration. Morphological changes in brain tissues were observed using HE and Nissl staining.
Results |
PG significantly reduced colistin-induced neuronal apoptosis levels. The apoptosis-related protein expressions were suppressed after PG intervention. Mechanistically, PG increased the levels of antioxidant factors and decreased the levels of oxidative factors, which might be related to the activation of the NRF2 pathway. In addition, PG treatment reversed the deviations in mitochondrial morphology and membrane potential. PG suppressed autophagy levels in N2a cells, possibly because PG inhibited colistin-induced apoptosis, thus reducing the level of spontaneous protective autophagy in cells. Nrf2 knockdown N2a cell models were applied to confirm that the activation of the NRF2 pathway played a vital role in PG alleviating the nerve damage caused by colistin.
Conclusion |
PG is a potential treatment option for colistin-induced neurotoxicity. It mitigated colistin-induced oxidative stress-associated injury and mitochondrial damage by activating the NRF2/HO-1 pathway, thus reducing nerve cell apoptosis.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Abbreviations : PG, Cyt c, SOD, CAT, GSH, MDA, Keap1, NRF2, HO-1, Bcl-2, Bax, MEM
Keywords : Colistin, Neurotoxicity, Piceatannol-3′-O-β-D-glucopyranoside (PG), Apoptosis, NRF2, Autophagy
Plan
Vol 161
Article 114419- mai 2023 Retour au numéro
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