Vasoactive intestinal peptide is involved in the inhibitory effect of interleukin-1ß on the jejunal contractile response induced by acetylcholine - 06/05/08
Anne-Catherine Aubé [1],
Christine Cherbut [1],
Claude Rozé [2],
Jean-Paul Galmiche [1]
Voir les affiliationsAlthough previous studies have shown that interleukin-1ß (IL-1ß) decreases acetylcholine (ACh)-induced intestinal contraction by an action on the enteric nervous system, the neuromediator(s) involved are still unknow.
Aim |
To determine the role of nitric oxide (NO), vasoactive intestinal peptide (VIP) and/or adenosine triphosphate (ATP) in mediating this inhibitory effect.
Methods |
The effects of NO synthase inhibitors, VIP and ATP antagonists on motor response to the ACh were investigated before and after 90-min exposure of a rat preparation of jejunal longitudinal muscle-myenteric plexus to IL-1ß. Ng-nitro-L-arginine methyl ester, Ng-nitro-L-arginine and Ng-monomethyl-L-arginine were used to inhibit NO synthase, VIP (10-28) and [D-p-Cl-Phe 6 , Leu 17 ] VIP to block VIP receptors, and suramin to block ATP receptors.
Results |
NO synthase inhibitors failed to block the inhibitory effect of IL-1ß on ACh-contracted jejunum smooth muscle. Suramin also failed to affect IL-1ß-induced inhibition, whereas VIP antagonists abolished it. Moreover, the action of IL-1ß was partly reproduced by VIP.
Conclusions |
While neither NO nor ATP accounts for the inhibitory effect of IL-1ß on ACh-contracted jejunum, VIP seems to be a key-mediator of this effect.
Keywords:
Interleukin-1ß
,
Intestinal motility
,
Vasoactive intestinal peptide
,
Rat
,
In vitro
Plan
© 2001 Elsevier Masson SAS. Tous droits réservés.
Vol 25 - N° 12
P. 1090-1095 - décembre 2001 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.