A study on differentially expressed gene screening of Chrysanthemum plants under sound stress - 14/05/08

Doi : 10.1016/j.crvi.2008.02.007

Shao Hongbo ^a,^b,^c,^⁎,^{^{1
Li Biao and Shao Hongbo are the co-first authors of this paper because of equal contribution.}} , Li Biao ^d,^e,^⁎,^{^{1
Li Biao and Shao Hongbo are the co-first authors of this paper because of equal contribution.}} , Wang Bochu ^d,^e, Tang Kun ^d,^e, Liang Yilong ^d
^a State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences, Northwest A&F University, Yangling 712100, China
^b Binzhou University, Binzhou 256603, China
^c Institute of Life Sciences, Qingdao University of Science & Technology, Qingdao 266042, China
^d College of Bioinformatics, Chongqing University of Posts & Telecommunications, Chongqing 400065, China
^e Key Laboratory for Biomechanics & Tissue Engineering under the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China

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Abstract

Environmental stress can induce differential expression of genes of flower plants. It had been found that sound stimulation had an obvious effect on the growth and development of flower plants, but it is not reported on the differentially expressed genes and their expressing characteristics under sound stimulation. This is one of the few reports in terms of using the DDRT-PCR technique for screening the differentially expressed cDNA fragments responding to sound-wave stress on Chrysanthemum. Six differentially expressed cDNA fragments were obtained. Molecular weight of fragments was from 200 to 600 bp, respectively. Among differential fragments acquired, three of them (SA3, SG7–1, and CA2) were found to be positive fragments by northern dot hybridization, whose molecular weight are 270, 580 and 370 bp, respectively. SA3 was differentially expressed and SG7–1 was preferably expressed, while CA2 was restrained by the sound wave. These results indicated that expression of some genes was turned on, meanwhile the stress restrained some genes from expression under the mode of sound-stress stimulation. To cite this article: S. Hongbo et al., C. R. Biologies 331 (2008).

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Keywords : DDRT-PCR (Differentially Display Reverse Transcription-Polymerase Chain Reaction), Northern dot hybridization, Sound stress, Physiological response, Chrysanthemum