I012 Transforming growth factor-beta activity in macrophages protects from angiotensin ii-induced aortic aneurysm in mice - 17/04/09
Résumé |
Background |
Complicated aortic aneurysm is a major cause of mortality in elderly men. The critical pathophysiological mechanisms responsible for disease development and complications remain largely unknown. Mutations in transforming growth factor (TGF)-β receptor type II are associated with familial forms of the disease, and increased angiotensin II (AII)-dependent TGF-β activity has been directly linked to aortic aneurysm formation in a mouse model of Marfan syndrome. However, the direct role of TGF-β signaling in common forms of the disease has not been assessed.
Methods and Results |
AII-induced abdominal aortic aneurysm (AAA) is a validated model of aneurysm formation in mice, and is prevented, like murine Marfan syndrome, by treatment with angiotensin II receptor type 1 antagonists. Normocholesterolemic C57Bl/6 mice are resistant to AII-induced AAA. Here, we show that systemic neutralization of TGF-β activity leads to unexpected and marked increase in the susceptibility of these mice to AII-induced AAA (from 10 % to 92.5 %, n=40). These AAA display a large spectrum of complications on echography, including thrombosis, fissuration, false channel formation and rupture, leading to a high level of mortality (65 %). Unexpectedly, the disease and its complications were refractory to inhibition of IFN-γ (n=10, 100 % AAA), deletion of IL-4 (n=10, 80 % AAA) or deletion of T and B lymphocytes (n=22 Rag-/- mice, 77.5 % AAA). Interestingly, depletion of circulating monocytes for 14 days using clodronate liposomes completely prevented AAA formation (0 % AAA in clodronate group vs 60 % AAA at day 14 in the group without clodronate).
Conclusions |
This study identifies a major protective effect of TGF-β activity against AngII-induced AAA, through modulation of monocyte/ macrophage function.
Le texte complet de cet article est disponible en PDF.Vol 102 - N° S1
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