Médecine

Paramédical

Autres domaines


S'abonner

Experimental study of tendon healing early phase: Is IGF-1 expression influenced by platelet rich plasma gel? - 07/06/10

Doi : 10.1016/j.otsr.2010.03.010 
D.N. Lyras a, , K. Kazakos a, G. Agrogiannis b, D. Verettas a, A. Kokka b, G. Kiziridis a, E. Chronopoulos c, M. Tryfonidis a
a Democritus University Hospital of Thrace, Department of Orthopaedic and Trauma Surgery, Alexandroupolis, 84100 Dragana, Greece 
b National and Kapodistrian University of Athens, Greece, 1st Department of Pathology, School of Medicine, 75, Mikras Asias street, 11527, Greece 
c Konstantopoulion University Hospital, Nea Ionia, Athens, Greece 

Corresponding author. Argyrokastrou 31B, 15235 Vrilissia, Athens, Greece. Tel.: +30 6949313155.

Bienvenue sur EM-consulte, la référence des professionnels de santé.
Article gratuit.

Connectez-vous pour en bénéficier!

Summary

Background

It is well established that growth factors play a critical role in the healing process of connective tissues. To our knowledge, there are no studies in literature concerning the influence of PRP on growth factors expression.

Hypothesis

The aim of this study was to assess the effect of a single application of platelet rich plasma (PRP) gel in a patellar tendon defect on the spatial and temporal expression of Insulin-like Growth Factor 1 (IGF-1) during tendon healing.

Materials and methods

Twenty-four animals were randomized to receive PRP (PRPFast, Bioteck) in a gel form (PRP group) and 24 to serve as untreated controls (Control group). A defect of 3mm×10mm was surgically created on the tendon under general anaesthetic and in the PRP group, PRP gel was applied to fill the tendon defect whereas no treatment was applied in the control group. Six animals (12 limbs) from each treatment-group were sacrificed after one, two, three and four weeks following treatment. Histological and immunohistochemical staining were performed.

Results

Histology revealed a faster healing process in the tendons of PRP group in comparison with the controls. In the first 2 weeks of healing, IGF-1 was found intracellularly in various type cells, whereas in the last 2 weeks of healing, IGF-1 was detected mainly in tenocytes. Both cytoplasmic and nuclear expressions were present, whereas the larger amounts of immunoexpression were localized in both epitenon and endotenon. A superior expression of IGF-1 was seen in PRP group compared with controls (p<0.0001) in both the epitenon and endotenon at each time point except at 4th week of healing where a superior expression of IGF-1 was shown in the endotenon of control group, compared to the PRP group (p<0.0001).

Conclusion

PRP may improve tendon defect healing by overexpression of IGF-1.

Level of evidence

Laboratory control animal study.

Le texte complet de cet article est disponible en PDF.

Keywords : Platelet rich plasma, Tendon healing, Immunohistochemistry, Growth factors, IGF-1


Plan


© 2010  Publié par Elsevier Masson SAS.
Ajouter à ma bibliothèque Retirer de ma bibliothèque Imprimer
Export

    Export citations

  • Fichier

  • Contenu

Vol 96 - N° 4

P. 381-387 - juin 2010 Retour au numéro

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

;

Mon compte


Plateformes Elsevier Masson

Déclaration CNIL

EM-CONSULTE.COM est déclaré à la CNIL, déclaration n° 1286925.

En application de la loi nº78-17 du 6 janvier 1978 relative à l'informatique, aux fichiers et aux libertés, vous disposez des droits d'opposition (art.26 de la loi), d'accès (art.34 à 38 de la loi), et de rectification (art.36 de la loi) des données vous concernant. Ainsi, vous pouvez exiger que soient rectifiées, complétées, clarifiées, mises à jour ou effacées les informations vous concernant qui sont inexactes, incomplètes, équivoques, périmées ou dont la collecte ou l'utilisation ou la conservation est interdite.
Les informations personnelles concernant les visiteurs de notre site, y compris leur identité, sont confidentielles.
Le responsable du site s'engage sur l'honneur à respecter les conditions légales de confidentialité applicables en France et à ne pas divulguer ces informations à des tiers.