Immunohistochemical staining of lentigo maligna during Mohs micrographic surgery using MART-1 - 01/09/11
Abstract |
Background: Lentigo maligna (LM) often displays extensive subclinical spread. Mohs micrographic surgery (MMS) has been proposed to help delineate the true histologic margin; however, visualizing atypical melanocytes on frozen section is challenging and often requires confirmatory permanent paraffin sections. Objective: Our aim was to use a monoclonal antibody to rapidly stain frozen sections during MMS to facilitate better visualization of atypical melanocytes. Methods: Frozen sections of LM during MMS were stained with MART-1 (melanoma antigen recognized by T cells) and compared with paraffin-embedded sections. Results: We found 100% correlation between frozen sections stained with MART-1 and paraffin-embedded sections. Conclusions: Atypical melanocytes can be better visualized on frozen sections of LM by using MART-1 rather than hematoxylin and eosin. This allows for easier identification during MMS and better chance of complete removal of LM lesions. (J Am Acad Dermatol 2002;46:78-84.)
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Supported by the Dermatology Foundation at Beth Israel Deaconess Medical Center. |
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Conflicts of interest: None. |
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Reprints not available from authors. |
Vol 46 - N° 1
P. 78-84 - janvier 2002 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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