CD4+CD7− T cells compose the dominant T-cell clone in the peripheral blood of patients with Sézary syndrome - 02/09/11
Abstract |
Background: Absence of CD7 antigen expression in T cells defines a subset of normal CD4+ CD45RO+ CD45RA− memory cells and is furthermore observed in Sézary syndrome (SS). Objective: Our purpose was to identify circulating T-cell clones in patients with SS and to elucidate whether the dominant T-cell clones express the CD7 antigen. Methods: Peripheral blood lymphocytes of patients with SS were analyzed by two-color flow cytometry using antibodies to the Vβ region of the T cell receptor (TCR) in combination with an antibody to CD7. In addition, T cells were analyzed for TCR-γ gene rearrangement by polymerase chain reaction (PCR) techniques. Results: Clonal T-cell expansion was detected in 7 patients with SS by immunostaining of the TCR Vβ regions. PCR analysis confirmed the presence of dominant T cell clones. Double-immunostaining revealed that in each case cells of the clonal Vβ TCR rearrangement homogeneously express the CD4+CD7− phenotype. Furthermore, CD4+CD7− cells express the CD15s antigen but lack expression of CD26 and CD49d. Conclusion: Expansion of clonal T cells strongly correlates with the expansion of CD4+CD7− T cells in 7 tested patients with SS. This supports our model that a subset of late differentiated, normal CD4+CD7− memory T cells may represent the physiologic counterpart of Sézary cells. Monitoring of circulating T cells with the CD4+CD7−CD15s+CD26−CD49d− phenotype proved to be useful for the identification of clonal T cells in patients with SS. (J Am Acad Dermatol 2001;44:456-61.)
Le texte complet de cet article est disponible en PDF.Abbreviations : CTCL, mAb, MF, PBMC, PCR, SS, TCR
Plan
Supported by the Deutsche Forschungsgemeinschaft DFG (to U. R.; Re 690/4-2) and the Fritz-Bender Stiftung, Munich (to H. A.). |
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Reprint requests: Uwe Reinhold, MD, Department of Dermatology, The Saarland University Hospital, 66421 Homburg/Saar, Germany. |
Vol 44 - N° 3
P. 456-461 - mars 2001 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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