Efficient reactivation of latent herpes simplex virus type 1 infection by excimer laser keratectomy in the experimental rabbit ocular model - 09/09/11
Abstract |
Purpose |
To investigate the role of excimer laser keratectomy as a trigger for the reactivation of latent HSV type 1 (HSV-1) in the New Zealand rabbit ocular model. There are conflicting reports in the current literature about reactivation of HSV-1 after excimer laser photoablation.
Methods |
New Zealand rabbits were inoculated topically with HSV-1 McKrae or W strain in each eye, and culture-positive dendritic keratitis was documented on day 7. After the establishment of latency (21+ days), animals were divided into three groups: group I animals underwent excimer laser photoablation in each eye; group II animals received intrastromal injections of sterile water to act as positive controls (a standard method); and group III animals received no treatment and represented spontaneous shedders. All eyes were swabbed daily from days 1 through 10 and plated on A549 cells. Recovery of HSV-1 on days 1 through 10 postinduction was analyzed to compare the efficiency of the different methods of viral reactivation.
Results |
Reactivation of latent HSV-1 after excimer treatment was observed in nine (45%) of 20 eyes and was equivalent to the rate of reactivation seen in the positive control animals (eight [44.4%] of 18 eyes) (P =.99). Both of these rates were significantly greater than those of the untreated animals (one [5.6%] of 18 eyes) (P =.018).
Conclusion |
Excimer laser keratectomy appears to be an efficient trigger for the reactivation of latent HSV-1 in the New Zealand rabbit ocular model.
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Vol 125 - N° 4
P. 488-492 - avril 1998 Retour au numéro
Article précédent
- Experimental study of tetrodotoxin, a long-acting topical anesthetic
- Daniel M. Schwartz, Howard L. Fields, Keith G. Duncan, Jacque L. Duncan, Matthew R. Jones
- Long-term tamoxifen citrate use and potential ocular toxicity
- Michael B. Gorin, Richard Day, Joseph P. Costantino, Bernard Fisher, Carol K. Redmond, Lawrence Wickerham, Julius E.S. Gomolin, Richard G. Margolese, Mathen K. Mathen, David M. Bowman, David Kaufmann, Nikolay V. Dimitrov, Lawrence J. Singerman, Richard Bornstein, Norman Wolmark
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