The efficacy of C-phycocyanin obtained from Arthrospira maxima SAG 25780 was evaluated on the levels of lipid peroxidation, and antioxidant parameters as well as histopathological and immunohistochemical assessment in carbon tetrachloride (CCl₄) induced liver damage in the male Wistar albino rats. The rats were injected by CCl₄ intraperitoneal (50% CCl₄, 0.5mg/kg body weight) that led to a marked increase in oxidative stress indicated by alterations in lipid peroxidation, enzymic and non-enzymic antioxidants. Treatment with C-phycocyanin (75mg/kg body weight) restored the above-mentioned alterations towards normalcy, highlighting the antioxidant potential of C-phycocyanin in mitigating the oxidative stress mediated damage produced during CCl₄-induced male rats. The liver damage was further confirmed by histopathological and immunohistochemical studies. The present investigation strongly suggested that the C-phycocyanin protects CCl₄-induced hepatotoxicity in Wistar rats.