Identification and genotyping of human papillomavirus in a Spanish cohort of penile squamous cell carcinomas: Correlation with pathologic subtypes, p16INK4a expression, and prognosis - 14/12/12
, Emili Masferrer, MD e, f, Ines de Torres, MD b, Belen Lloveras, MD g, i, Javier Hernandez-Losa, PhD b, Sergio Mojal, BMS h, Carlos Salvador, MD c, Juan Morote, MD c, Santiago Ramon y Cajal, MD b, Ramon M. Pujol, MD f, Vicente Garcia-Patos, MD a, d, Agustin Toll, MD d, f
Reprint requests: Carla Ferrándiz-Pulido, MD, Department of Dermatology, Hospital Universitari Vall d’Hebron, Passeig Vall d’Hebron 119-129, 08035 Barcelona, Spain.Abstract |
Background |
Penile squamous cell carcinoma (PSCC) is a tumor with a high metastatic potential. In PSCC the attributable fraction to human papillomavirus (HPV) is not well established.
Objective |
We sought to provide novel data about the prevalence of HPV in a large series of penile intraepithelial neoplasia (PeIN) and invasive PSCC, correlating the results with the histologic subtype, p16INK4a immunostaining, and prognosis.
Methods |
A total of 82 PSCC were included in the study, 69 invasive and 13 PeIN. HPV detection was performed by polymerase chain reaction with SPF-10 broad-spectrum primers followed by DNA enzyme immunoassay and genotyping with a reverse hybridization line probe assay. P16INK4a immunohistochemical expression on tissue microarrays was also analyzed.
Results |
HPV DNA was identified in 31 of 77 (40.2%) PSCC (22 of 67 invasive and 9 of 10 PeIN). In 25 of 31 (80.6%) cases HPV-16 was identified. HPV detection was significantly associated with some histologic subtypes: most basaloid and warty tumors were high-risk HPV (hrHPV) positive, whereas only 15% of usual PSCC were hr-HPV positive. All hrHPV-positive PSCC had an adjacent undifferentiated PeIN. Strong p16INK4a immunostaining correlated with hrHPV infection. Most undifferentiated PeIN showed p16INK4a immunohistochemical overexpression. Both hrHPV-positive and p16INK4a-positive tumors showed a better overall survival without reaching statistical significance.
Limitations |
This was a retrospective study.
Conclusions |
Our results suggest that most hrHPV-positive PSCC develop from undifferentiated hrHPV-positive PeIN. P16INK4a immunostaining may be useful in identifying both etiologically related hrHPV-positive tumors and those with better outcome. The routine use of p16INK4a staining should be incorporated in histologic evaluation of PSCC.
Le texte complet de cet article est disponible en PDF.Key words : human papillomavirus, p16INK4a, penile intraepithelial neoplasia, penis, squamous cell carcinoma, survival
Abbreviations used : adjPeIN, HPV, hrHPV, PeIN, PSCC, SCC
Plan
| Dr Toll and Garcia-Patos equally contributed to the work. |
|
| Supported in part by the grants PI04/1728, RD09/0076/00036, and PI10/00785 from Fondo de Investigación Sanitaria, Instituto de Salud Carlos III, Ministerio de Sanidad, Federación Española de Enfermedades Raras (FEDER), Spain. The funder was not involved in study design, data collection, data analysis, manuscript preparation, or publication decisions. |
|
| Conflicts of interest: None declared. |
Vol 68 - N° 1
P. 73-82 - janvier 2013 Retour au numéro
Article précédent
- Patient-reported reasons for the discontinuation of commonly used treatments for moderate to severe psoriasis
- Howa Yeung, Joy Wan, Abby S. Van Voorhees, Kristina Callis Duffin, Gerald G. Krueger, Robert E. Kalb, Jamie D. Weisman, Brian R. Sperber, Bruce A. Brod, Stephen M. Schleicher, Bruce F. Bebo, Daniel B. Shin, Andrea B. Troxel, Joel M. Gelfand
- Epidermolysis Bullosa Oropharyngeal Severity (EBOS) score: A multicenter development and reliability assessment
- Giulio Fortuna, Nita Chainani-Wu, Francina Lozada-Nur, Massimo Aria, Rodrigo Cepeda-Valdes, Annamaria Pollio, M. Peter Marinkovich, Adriana E. Martinez-Salazar, Michele D. Mignogna, Anna L. Bruckner, Julio Cesar Salas-Alanís
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?