Immunomodulatory activity of isoflavones isolated from Iris kashmiriana: Effect on T-lymphocyte proliferation and cytokine production in Balb/c mice - 15/06/13
Abstract |
This study deals with the evaluation of immunomodulatory action of a new isoflavone isonigricin (1) and first report isoirisolidone (2) isolated from rhizomes of Iris kashmiriana. Flowcytometric method was employed to study their effect on production of T-lymphocytes (CD4+ and CD8+ T-cells) and T-cell cytokines (IL-2, IL-4 and IFN-γ) in a dose-dependant manner. Drugs at doses of 0.025 to 0.8mg/kg per oral dose showed compound 1 to possess immunosuppressant activity on T-cells as well as cytokines, while compound 2 acted as immunostimulator for both cells and cytokines. However, their methylated products viz. 1OMe and 2OMe exhibited stimulatory effect on interleukins under study and suppressive effect on production of T-lymphocytes and IFN-γ. The oral LD50 in mice was observed to be more than 200mg/kg body weight. Such a difference in their activity is the manifestation of the position and nature of substituents present on isoflavonoidal skeleton and demonstrates the potential of these isolates as prospective immunomodulatory agents.
Le texte complet de cet article est disponible en PDF.Keywords : Iris kashmiriana, Iridaceae, Isoflavones, Cytokines, Immunomodulators, Flowcytometry
Plan
Vol 3 - N° 2
P. 151-157 - avril 2013 Retour au numéro
Article précédent
- Biochemical characterization of proteins and enzymes of Bungarus caeruleus venom
- Sheeja S. Rajan, Albert Rajendran, Bannikuppe S. Viswanath
- Luteolin a dietary flavonoid attenuates isoproterenol-induced myocardial oxidative stress in rat myocardium: An in vivo study
- Murugesan Madhesh, Manju Vaiyapuri
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?