Flow Cytometry in Mastocytosis : Utility as a Diagnostic and Prognostic Tool - 17/04/14
Doi : 10.1016/j.iac.2014.01.008
Laura Sánchez-Muñoz, MD, PhD a, b, ⁎ 
, Cristina Teodosio, PhD b, c, Jose Mario T. Morgado, MSc a, b, Omar Perbellini, MD d, Andrea Mayado, PhD b, c, Ivan Alvarez-Twose, MD a, b, Almudena Matito, MD, PhD a, b, María Jara-Acevedo, MSc b, c, Andrés C. García-Montero, PhD b, c, Alberto Orfao, MD, PhD b, c, Luis Escribano, MD, PhD b, c
, Cristina Teodosio, PhD b, c, Jose Mario T. Morgado, MSc a, b, Omar Perbellini, MD d, Andrea Mayado, PhD b, c, Ivan Alvarez-Twose, MD a, b, Almudena Matito, MD, PhD a, b, María Jara-Acevedo, MSc b, c, Andrés C. García-Montero, PhD b, c, Alberto Orfao, MD, PhD b, c, Luis Escribano, MD, PhD b, c a Mastocytosis Unit of Castilla La-Mancha, Hospital Virgen del Valle, Toledo, Spain
b Spanish Network on Mastocytosis (REMA), Toledo, Spain
c Departments of Cytometry and Medicine, Centro de Investigación del Cáncer, (IBMCC-CSIC/USAL and IBSAL) University of Salamanca, Salamanca, Spain
d Section of Hematology, Department of Medicine, University of Verona, Verona, Italy
∗Corresponding author. Mastocytosis Unit of Castilla La-Mancha, Hospital Virgen del Valle, Toledo, Spain.
Résumé |
This article presents information for the identification and characterization of mast cells from bone marrow and other tissues using multiparametric flow cytometry. In addition, it provides guidelines for the application of this technique in the subclassification of systemic mastocytosis and assessment of the long-term prognosis of patients individually.
Le texte complet de cet article est disponible en PDF.Keywords : Mast cells, Mastocytosis, Flow cytometry, Bone marrow, Cell purification, Diagnosis, Classification, Prognosis
Plan
| No potential conflicts of interest. |
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| This work was supported by grants from the Fondo de Investigaciones Sanitarias (FIS) of the Instituto de Salud Carlos III, Ministerio de Economía y Competitividad of Spain (PS09/00032); Fundación Sociosanitaria de Castilla-La Mancha (2010/008); Fundación Española de Mastocitosis (FEM 2013). |
© 2014
Elsevier Inc. Tous droits réservés.
Vol 34 - N° 2
P. 297-313 - mai 2014 Retour au numéro
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