Ulcerative colitis (UC) is associated with tainted neutrophil infiltration, deregulated pro-inflammatory mediators, characterized by severe oxidative stress of the intestine. In the present study, an effort was made to evaluate the effect of methanolic fractions of Eupatorium triplinerve (E. triplinerve) (ET) on acetic acid induced ulcerative colitis in male adult mice.

Colitis in mice was induced with 3.0% acetic acid (v/v) in saline via rectal route. Pre-intervention with E. triplinerve extract (100mg and 200mgkg⁻¹ body weight, oral) and reference drug ranitidine (50mgkg⁻¹ body weight used as reference, oral) 4days before induction of colitis and was extended up to 8days.

The phase of inflammation before E. triplinerve extract pre-treatment significantly showed attenuated macroscopic damage, argyrophilic nuclear organization regions (AgNORs) count and histological changes. Similarly, extract also effectively detracts the activity of both Myeloperoxidase (MPO) and Malondialdehyde (MDA) levels by enhancing the cellular antioxidant enzyme levels (Glutathione-s-transferase [GST], Glutathione peroxidise [GPx] and Catalase [CAT]) at the site of ulceration.

The E. triplinerve based therapy resolved that some constituents in extract have an antiulcer effect against UC at colon specific area through its inviolable radical scavenging activity.