Urease positive probiotic Lactobacillus strains were tested for oxidative stress and uremic profile on experimental rat (Wister strains) induced by acetaminophen (APAP) overdose. Experimental rats received acetaminophen interperitoneally at the dose of 500mg/kg/day, continuously for 10days. From 11th day onwards they were orally fed with Lactobacillus fermentum (MTCC 903), Lactobacillus plantarum (MTCC 4462) and Lactobacillus rhamnosus (MTCC 1408) respectively at the dose of 10⁹ CFU/mL/100g of body weight/day for 15days continuously. Plasma, kidney, liver and fecal samples were tested for uremic profile of the sacrificed rats after the experiment. In APAP treated rats, plasma urea, creatinine (Cr), glutamate oxaloacetate transaminase (GOT) and malonaldehyde (MDA) level elevated and catalase (CAT) and super oxide diusmutase (SOD) level declined significantly compared to negative control. However, level of plasma urea, Cr, GOT and MDA in tested rats were significantly lower in comparison to positive control. The uremic profile of the probiotic induced rats was very much comparable with the negative control, even better for some parametric values. Prevention of DNA fragmentation in kidney tissues and reduction of enteric pathogens in feces of Lactobacillus fed rats were noticed. Electrolytes profile of the tested plasma samples were in acceptable range. To sum up, tested urease positive Lactobacillus strains were shown to improve the clinical condition of the acetaminophen induced uremic experimental rats.