Cytotoxicity induction by ethanolic extract of Acalypha indica loaded casein-chitosan microparticles in human prostate cancer cell line in vitro - 13/08/14
Abstract |
The present exploration reports an extensive evaluation of in vitro anticancer efficacy of a novel aqueous ethanolic extract of Acalypha indica (ETAI) loaded chitosan-casein (CS-CT) microparticles in a cancer cell line model. Spherically shaped ETAI loaded CS-CT (CS/CT/ETAI) microparticles were prepared by colloidal coacervation technique in a completely aqueous environment with an entrapment efficiency and particle size of 85.30±4.10 and 2±0.96μm respectively. Cytotoxicity, as investigated on human prostate cancer cell line (PC3) by MTT assay, revealed insignificant differences between free ETAI and CS/CT/ETAI microparticles treated cells in the first 24h, while higher cytotoxicity was demonstrated by CS/CT/ETAI microparticles, following 72h of incubation. Percentage of cytotoxicity also demonstrated by LDH assay, which shown the concentration dependent leakage of LDH from PC3 cells exposed to free ETAI and CS/CT/ETAI microparticles The use of significantly low concentration of Acalypha indica loaded with CS/CT is a much better approach in comparison to the use of free ETAI for cancer treatment in future.
Le texte complet de cet article est disponible en PDF.Keywords : Casein, Chitosan, Microparticles, Acalypha indica, PC3 cells
Plan
Vol 4 - N° 3
P. 445-450 - juillet 2014 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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