Hypericin biosynthesis in Hypericum hookerianum Wight and Arn: Investigation on biochemical pathways using metabolite inhibitors and suppression subtractive hybridization - 02/10/14
, Aswati R. Nair bBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 10 |
| Iconographies | 7 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Abstract |
The biochemical pathway to hypericin biosynthesis is presumed to be polyketide synthase (PKS) mediated, but it has not been experimentally validated, and no alternate route (chorismate/o-succinylbenzoate pathway) has been analyzed. We report here our earlier developed auxin inducible culture systems of Hypericum hookerianum as a model, to study the metabolic pathway to hypericin synthesis. Inhibitors of the alternate pathway at varying concentrations showed steady synthesis of total hypericins with means of 2.80±0.22, 18.75±0.01; 16.39±3.75, 29.60±1.90 (mevinolin) 2.53±0.10, 18.12±0.56; 0.14±0.01, 14.28±1.11 (fosmidomycin) and 2.7±0.35, 18.75±0.61; 0.14±0.01, 12.80±1.09mgg−1 DW (glyphosate) in the control and auxin-induced shoot and shoot-forming callus cultures, respectively. SSH analysis classified the differentially expressed sequences into protein synthesis (38%), modification (20%), electron transport (9%) and remaining as unclassified (11%) and unknown proteins (22%). Functional annotation of sequences indicates the presence of additional protein components besides PKS activity. Our results demonstrate direct biochemical and molecular evidence of PKS hypothesis of hypericin biosynthesis for the first time.
Le texte complet de cet article est disponible en PDF.Keywords : Hypericum hookerianum, Hypericin, Mevinolin, Fosmidomycin, Glyphosate, Type III PKS, SSH
Abbreviations : AQ, BPS, CHS, DW, FW, Kin, NAA, OKS, PGR, PKS, SSH
Plan
Vol 337 - N° 10
P. 571-580 - octobre 2014 Retour au numéro
Article précédent
- Haloperidol treatment at pre-exposure phase reduces the disturbance of latent inhibition in rats with neonatal ventral hippocampus lesions
- Zakaria Ouhaz, Saadia Ba-M’hamed, Mohamed Bennis
- Potential lactoferrin activity against pathogenic viruses
- Elrashdy M. Redwan, Vladimir N. Uversky, Esmail M. El-Fakharany, Hussein Al-Mehdar
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?