Detection of mitotic figures in thin melanomas—Immunohistochemistry does not replace the careful search for mitotic figures in hematoxylin-eosin stain - 14/09/15
Abstract |
Background |
The mitotic rate is an important prognostic criterion in patients with thin melanoma ≤ 1 mm.
Objective |
The aim of this study was to investigate the reproducibility of the mitotic rate in thin melanoma in hematoxylin-eosin (H&E) stain and compare it with the detection of mitotic figures by immunohistochemistry.
Methods |
The number of mitoses stated in the routine diagnostic report in 190 pT1 melanomas was compared with the number gained from re-evaluation of H&E sections and the number detected after staining with the mitotic marker, phosphohistone H3 (PHH3). Two different approaches were used for choosing the “hot spot” for evaluation (dermal vs epidermal/dermal).
Results |
Comparing routine H&E-stained slides with re-evaluation slides, the number of mitotic figures was slightly variable. However, findings did not result in a change of the tumor stage. In 34% of the tumors with dermal mitotic figures on H&E, mitoses could not be found in the corresponding PHH3 slide anymore. In 4% of the cases, stage relevant mitoses could only be found by PHH3 immunohistochemistry.
Limitation |
This is a single center study.
Conclusion |
Immunohistochemical staining for mitotic figures does not replace a careful evaluation of H&E-stained slides. Immunohistochemical detection of mitosis is only an additional tool; the time-saving effect is therefore negligible.
Le texte complet de cet article est disponible en PDF.Key words : hot spot, immunohistochemistry, mitosis, mitotic rate, phosphohistone H3, pT1, thin melanoma
Plan
Funding sources: None. |
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Parts of this work were presented as a poster at the meeting of the Dermatohistopathologic Working Group German Society of Dermatology, 2014. |
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Conflicts of interest: None declared. |
Vol 73 - N° 4
P. 637-644 - octobre 2015 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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