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Archives of cardiovascular diseases
Volume 109, n° 8-9
pages 465-475 (août 2016)
Doi : 10.1016/j.acvd.2016.01.015
Received : 21 July 2015 ;  accepted : 27 January 2016
Impact of percutaneous pulmonary valve implantation procedural steps on leaflets histology and mechanical behaviour: An in vitro study
Analyse de l’impact des manipulations pré-implantatoires sur la structure histologique et le comportement mécanique des feuillets valvulaires des prothèses pulmonaires percutanées : étude in vitro

Figure 1

Figure 1 : 

Description of in vitro manipulations to the Melody® valved stent. A. Melody® valved stent after rinsing with saline solution. B and C. Crimping on sterile 5-mL and 2.5-mL syringes. D. Crimping on the Ensemble® delivery system. E. Covering of the valved stent by the sheath. F. Expansion of the valved stent in a 20-mm GORE-TEX® conduit. G. Post-dilatation using high-pressure balloon. H. Bovine jugular vein detached from the stent and opened on its longitudinal axis (note the three valvular leaflets). I. One valvular leaflet after sampling.

Figure 2

Figure 2 : 

Histological observation of Melody® valvular leaflets (haematoxylin and eosin staining; ×5 magnification). A. Microscopic aspect of a control valvular leaflet (condition I) with a conserved architecture. B. Microscopic aspect of an injured valvular leaflet (condition III: expansion); note the inhomogeneous architecture associated with a large transverse fracture (*).

Figure 3

Figure 3 : 

Histological observation of bovine (A and B) and porcine (C and D) pericardial leaflets (haematoxylin and eosin staining; ×5 magnification). A. Microscopic aspect of a control bovine pericardial valvular leaflet (condition I) with a preserved organization of collagen bundles. B. Microscopic aspect of a traumatized bovine pericardial valvular leaflet (condition IV: post-dilatation); note the complete architecture alteration associated with a large transverse fracture (*). C. Microscopic aspect of a control porcine pericardial valvular leaflet (condition I) with a slightly altered architecture. D. Microscopic aspect of an injured porcine pericardial valvular leaflet (condition III: expansion); note the presence of a large transverse fracture (*).

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