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Biomedicine and pharmacotherapy
Volume 87
pages 621-627 (mars 2017)
Doi : 10.1016/j.biopha.2016.12.121
Received : 28 October 2016 ;  accepted : 27 December 2016
Original Articles

MiR-451 suppresses proliferation, migration and promotes apoptosis of the human osteosarcoma by targeting macrophage migration inhibitory factor
 

Wei Liu a, b, 1, Sheng-Yao Liu a, 1, Yong-Bin He a, Rui-Liang Huang b, Song-Yun Deng a, Guo-Xin Ni a, c, 2, , Bin Yu a, 2,
a Department of Orthopeadics and Traumatology, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue (N), Guangzhou 510515, China 
b Department of Orthopedics, The People's Hospital of Gaoming District of Foshan City, Guangdong 528500, China 
c Department of Rehabilitation Medicine, First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou 350005, China 

Corresponding authors at: Department of Orthopeadics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.Department of Orthopeadics and TraumatologyNanfang HospitalSouthern Medical University1838 Guangzhou Avenue (N)Guangzhou510515China
Graphical abstract




The full text of this article is available in PDF format.
Abstract

Previous studies have shown that MiR-451 plays an important role in human osteosarcoma carcinogenesis, but the underlying mechanism by which MiR-451 affects the osteosarcoma has not been fully understood. This study intends to uncover the mechanism by which MiR-451 functions as a tumor suppressor. The expression of MiR-451 in osteosarcoma tissues and osteosarcoma cell lines was monitored by real-time PCR. The proliferation ability was examined by MTT and cell cycle assay. The migration and apoptosis of cells were monitored by migration assay and flow cytometry, respectively. Moreover, the angiogenesis of HUVEC cells transfected with MiR-451 mimics was examined by tube formation assay. The effect of MiR-451 on MIF was determined by luciferase assays and Western blot assay. The results showed that MiR-451 expression level was significantly reduced in the osteosarcoma compared with normal bone tissues. Overexpression of MiR-451 significantly attenuated the proliferation and migration, and induced the apoptosis of osteosarcoma cells. Furthermore, the angiogenesis of HUVEC cells transfected with MiR-451 mimics was assayed and the decreased angiogenic ability was detected compared to the controls. Finally, we demonstrated that MiR-451 overexpression inhibited the malignant behavior of osteosarcoma by downregulating MIF. These findings suggest that MiR-451 may act as a tumor suppressor in osteosarcoma. MiR-451 inhibited cell proliferation, migration and angiogenesis and promoted apoptosis of human osteosarcoma cells, at least partially, by inhibiting the expression of MIF. MiR-451/MIF may be a novel therapeutic target in treatment of osteosarcoma.

The full text of this article is available in PDF format.

Keywords : MiR-451, Proliferation, Migration, Apoptosis, MIF, Osteosarcoma


1  These authors should be regarded as co-first authors.
2  These authors contributed to the work equally and should be regarded as co-corresponding authors.


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