A membrane protein preferentially expressed by a subpopulation of immature lymphoid cells, epidermal basal keratinocytes, and other epithelial cells - 06/10/17
Résumé |
A murine monoclonal antibody, designated EL-1, was raised by immunization with a human malignant T cell line. It reacted specifically with a membrane antigen expressed on T and B lymphoblastoid cell lines, a subpopulation of normal thymocytes and bone marrow lymphocytes, lymphocytes from a subset of patients with non-B, non-T cell acute lymphoblastic leukemia or T cell acute lymphoblastic leukemia and epithelial stern cells. The latter reactivity was especially striking in the skin, where only basal epidermal keratinocytes and epidermal appendages, including eccrine sweat glands, sebaceous glands and hair follicles, stained positively. A human epidermoid carcinoma cell line was also stained by EL-1. Suprabasilar keratinocytes and acellular keratin did not stain. However, in vitro proliferating fetal lung fibroblasts stained positively. Membrane immunoprecipitation analysis demonstrated that the antigen recognized by antibody EL-1 is a single protein of molecular weight 105 kilodaltoris which did not change with exhaustive chemical reduction. Metabolic iadiolabeling studies demonstrated that this protein is synthesized by the cell and not merely taken up from the culture medium. This antibody can be useful in studying keratinocyte differentiation in epidermal malignancies and normal skin.
Le texte complet de cet article est disponible en PDF.Abbreviations used : ALL, AML, ATL, cALLA, CLL, CML, Con A, DNP, ELISA, ETAF, FCS, PHA, PMA, PWM, RPMI, SALT, SDS-PAGE, SS, Tdt
Supported in part by Public Health Service Grant No. AI-19080. A. B. G. is a recipient of a John A. and George L. Hartford Fellowship Award. The work was also partly supported by a grant from the National Institutes of Health to the Rockefeller University General Clinical Research Center. |
Vol 13 - N° 1
P. 54-65 - juillet 1985 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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