Anti-idiotype antibodies abrogate the tissue deposition of anti-RNP human autoantibodies injected into neonatal BALB/c mice - 01/01/02
Francisco Cabral-Arellano, Esperanza Avalos-Díaz, Sergio H. Sánchez-Rodríguez, Ricardo Villalobos, Rafael Herrera-Esparza * *Correspondence and reprints: Chepinque 306, Col. Lomas de la Soledad, Zacatecas, Zac, 98040, Mexico
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Résumé |
Objective. The goals of the current work are: 1) to examine the epidermal deposition of anti-RNP IgG human autoantibodies in neonatal BALB/c mice; 2) to look for immunoregulatory effects of anti-idiotypes allowing one to inhibit the epidermal deposition of anti-RNP antibodies; and 3) to elicit antinuclear antibodies in adult BALB/c mice by internal images of anti-idiotypes. Materials and methods; Anti-idiotype antibodies were produced with human anti-RNP IgG obtained by ion exchange chromatography; F(ab')2 fragments were recovered from pepsin digestion and were purified using Sephacryl S-300. F(ab')2 fragments were then used to immunize New Zealand rabbits. Results. The anti-RNP IgG recognized the 70 kDa protein and the A (31 kDa) and C (19 kDa) proteins, while the anti-idiotype antibody specifically recognized the light or heavy chain of the anti-RNP (Fab')2 fragments. Additionally, anti-idiotypes recognized the anti-RNP IgG from some sera, but not the IgG from other specificities or from normal IgG. When anti-RNP IgG was injected intraperitoneally into BALB/c mice it induced immune complex deposition in the epidermis and at the dermal-epidermal junction. Previous injection of anti-idiotype antibodies abrogated the anti-RNP IgG deposits. Vaccination with anti-idiotypes elicit antinuclear antibodies in adult BALB/c mice. Conclusions. Anti-idiotype antibodies abrogate in vitro the antinuclear antibody deposition in neonatal BALB/c mice. Anti-idiotype antibodies elicit antinuclear antibodies in adult BALB/c mice.
Mots clés : antinuclear antibodies ; anti-RNP antibodies ; idiotypic network ; immune complex ; lupus ; mixed connective tissue disease.
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Vol 69 - N° 1
P. 43-50 - janvier 2002 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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