Effect of cryoglobulin and crystalcryoglobulin on TNF- production by normal human monocytes - 01/01/02
pages | 4 |
Iconographies | 0 |
Vidéos | 0 |
Autres | 0 |
Résumé |
Objectives. We recovered an IgG1- cryocrystalglobulin in synovial fluid and membrane specimens from a patient with destructive arthropathy. In the present study, we investigated its proinflammatory properties by measuring its effects on TNF-α production by normal human monocytes. Materials and methods. Normal human monocytes isolated by plastic adhesion were cultured in microtiter plates. Adherent monocytes were cultured for 6, 8, and 24 hours with sterile cryocrystalglobulin (150 μg/mL and 2 mg/mL), type I noncrystallised cryoglobulin (same concentrations), monosodium urate (MSU) crystals (2 mg/mL), LPS (10 μg/mL), or medium alone. Supernatant TNF-α concentrations were assayed using an ELISA. Results. Cryocrystalglobulin had no effect on TNF-α production by normal human monocytes. Noncrystallised cryoglobulin increased TNF-α levels in supernatants in a time-dependent and concentration-dependent fashion. This increase was significantly less marked than the increases achieved with MSU crystals or LPS. Conclusion. IgG1 cryocrystalglobulin has no effect on TNF-α production by normal human monocytes. Fc region changes within the cryocrystalglobulin molecule may explain this finding.
Mots clés : cryoglobulin ; microcrystals ; monocytes ; TNF-α.
Plan
Vol 69 - N° 4
P. 379-382 - juin 2002 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?