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Biomedicine and pharmacotherapy
Volume 63, n° 9
pages 619-626 (novembre 2009)
Doi : 10.1016/j.biopha.2008.12.001
Received : 12 November 2008 ;  accepted : 6 December 2008
Gene expression, cytoskeletal changes and extracellular matrix synthesis in human osteoblasts treated with cyclosporin A
 

Maurizio Vertemati a, Ernesto Minola b, Claudia Dolci a, Giordano Stabellini a, , Furio Pezzetti c, Claudia Moscheni a, Carla Calastrini d, Manuela Bramerio b, Annalisa Palmieri e, Laura Vizzotto a
a Department of Human Morphology, University of Milan, Milan, Italy 
b Department of Pathological Anatomy Niguarda Hospital, Milan, Italy 
c Department of Histology, Embryology and Applied Biology, University of Bologna, Bologna, Italy 
d Department of Morphology and Embryology, Section of Histology and Embryology, University of Ferrara, Ferrara, Italy 
e Maxillofacial Surgery, University of Ferrara, Ferrara, Italy 

Corresponding author. Dipartimento di Morfologia Umana, Via Mangiagalli 31, 20133 Milano, Italy. Tel.: +39 025 031 5393; fax: +39 025 031 5387.
Abstract

Cyclosporin A (CyA) is an immunosuppressive agent used to prevent allograft rejection, but unfortunately it causes adverse effects such as bone diseases, osteoporosis and osteomalacia. These pathologies involve an imbalance between synthesis, degradation and mineralization of extracellular matrix. CyA can modify extracellular matrix components such as glycosaminoglycans (GAG) and collagen fibers. In addition, normal cell activity is dependent on cell morphology and substrate cell attachment. We treated normal human osteoblasts with CyA and analyzed: (i) gene expression by a microarray method; (ii) extracellular GAG and collagen after 3H-glucosamine and Western blot analysis; and (iii) cytoskeletal changes, using actin and tubulin fluorescent antibodies. CyA increased intra- and extracellular GAG and extracellular GAG classes such as hyaluronic acid, chondroitin sulphate, and dermatan sulphate; there was no noteworthy effect on heparan sulphate and the ratio of non-sulphated to sulphated GAG. In osteoblast cultures the drug reduced cytoskeletal actin, while tubulin did not change. In vivo the osteoblasts showed morphological changes with different extracellular matrix synthesis. Microarray analysis indicated the inhibition of gene pathways related to Wnt signaling molecules, and the cytoskeletal and focal adhesion cascade. In in vitro human osteoblasts CyA modified gene expression related to cytoskeletal pattern organization and cell morphology. Since in bone pathologies osteoblasts show different morphology related to cell size, these data suggest that in vivo osteoblast different functions could be dependent on alteration of osteoblast differentiation.

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Keywords : Cyclosporin A, Gene expression, Cytoskeleton




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