Article

PDF
Access to the PDF text
Advertising



Archives of cardiovascular diseases
Volume 104, n° 1
pages 17-28 (janvier 2011)
Doi : 10.1016/j.acvd.2010.09.006
Received : 26 February 2010 ;  accepted : 30 September 2010
Therapeutic effects of continuous infusion of brain natriuretic peptides on postmyocardial infarction ventricular remodelling in rats
Effet thérapeutique d’une perfusion continue de BNP sur le remodelage ventriculaire post-infarctus du myocarde chez le rat
 

Figure 1




Figure 1 : 

In panel A, a correlation curve was created for the blood troponin I level obtained from 21 MIs and infarction size was expressed as the percentage of necrosis/risk area, with a correlation coefficient of 0.979 (P <0.05). In panel B, Kaplan-Meier curves were created for the MI group, the MI+enalapril group, the MI+BNP group and the MI+combination therapy group. All the rats were rigorously monitored for evaluation of survival rate. Both enalapril and BNP infusion improved survival rate (P <0.05, respectively) but no further significant beneficial effects were obtained with combination therapy. *P <0.05 vs MI group. BNP: brain natriuretic peptide; CTnI: cardiac troponin I; MI: myocardial infarction.


Figure 2




Figure 2 : 

Effects of BNP, enalapril and combination therapy on Ang II (panel A), cGMP (panel B) and BNP (panel C) concentrations in non-infarcted left ventricular tissue. All concentrations are expressed as means±standard deviations. For comparison between groups at the same time point: *P <0.05 vs sham controls; §P <0.05 vs MI group; ¶, P <0.05 vs MI+enalapril group. For comparison within the MI group: #P <0.05 vs on day 3. Ang II: angiotensin II; BNP: brain natriuretic peptide; cGMP: cyclic guanosine monophosphate; MI: myocardial infarction.


Figure 3




Figure 3 : 

Representative bands for p-IκB⍺ vs total IκB⍺ (panel A), p-p65 subunit vs total p65 (panel B) and p-p50 subunit vs total p50 (panel C). The phosphorylation level of each subunit was then quantified and shown in a bar graph below, for each corresponding group. The expression levels of TNF⍺ (panel D), IL-1 (panel E) and IL-6 (panel F) were also quantified in bar graphs. All data are expressed as means±standard deviations. *P < 0.05 vs sham controls; §P < 0.05 vs MI group; ¶, P <0.05 vs MI+enalapril group; ΔP <0.05 vs MI+BNP group. ACEI: angiotensin-converting enzyme inhibitor; BNP: brain natriuretic peptide; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; IL: interleukin; MI: myocardial infarction; p: phosphorylated; TNF: tumour necrosis factor.


Figure 4




Figure 4 : 

The representative bands for MMP-2, MMP-9 and TIMP-1 are shown in panel A for day 3, panel B for day 7 and panel C for day 28. The expression level was quantified by densitometry and normalized to that of β-actin. The expression ratios were then calculated and shown in a bar graph for both MMP-2/TIMP-1 (panel D) and MMP-9/TIMP-1 (panel E) for day 7. All data are expressed as means±standard deviations. * P < 0.05 vs sham controls; §P < 0.05 vs MI group; ¶, P < 0.05 vs MI+enalapril group; ΔP < 0.05 vs MI+BNP group. ACEI: angiotensin-converting enzyme inhibitor; BNP: brain natriuretic peptide; MI: myocardial infarction; MMP: matrix metalloproteinase; TIMP: tissue inhibitor of metalloproteinase.


Figure 5




Figure 5 : 

The upper panel shows the representative zymographic imaging, with different bands to quantify the activities of MMPs. Pro-MMP-9, pro-MMP-2 and active MMP-2 are shown at 92kDa, 72kDa and 66kDa, respectively. Bar graphs in the lower panel demonstrate the activity of pro-MMP-2 at different time points (left) and the activity of pro-MMP-9 on day 3 (right). All data are expressed as means±standard deviations. For comparison between groups at the same time point: *P < 0.05 vs sham controls; §P < 0.05 vs MI group; ¶, P < 0.05 vs MI+enalapril group; ΔP < 0.05 vs MI+BNP group. B: MI+BNP group; BNP: brain natriuretic peptide; C: MI control group; E: MI+enalapril group; E+B: MI+combination group; MI: myocardial infarction; MMP: matrix metalloproteinase; S: sham control group.


Figure 6




Figure 6 : 

The effects of BNP and enalapril on CVF were quantified in the infarct area (panel A) and in non-infarcted left ventricular tissue (panel B). In non-infarcted left ventricular tissue, both collagen type I (panel C) and collagen type III (panel D) content were also quantified. For comparison between groups at the same time point: *P < 0.05 vs sham controls; §P < 0.05 vs MI group; ¶, P < 0.05 vs MI+enalapril group. For comparison within the MI group: #P < 0.05 on day 3; ΔP < 0.05 on day 7. BNP: brain natriuretic peptide; CVF: collagen volume fraction; MI: myocardial infarction.

EM-CONSULTE.COM is registrered at the CNIL, déclaration n° 1286925.
As per the Law relating to information storage and personal integrity, you have the right to oppose (art 26 of that law), access (art 34 of that law) and rectify (art 36 of that law) your personal data. You may thus request that your data, should it be inaccurate, incomplete, unclear, outdated, not be used or stored, be corrected, clarified, updated or deleted.
Personal information regarding our website's visitors, including their identity, is confidential.
The owners of this website hereby guarantee to respect the legal confidentiality conditions, applicable in France, and not to disclose this data to third parties.
Close
Article Outline