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Joint Bone Spine
Volume 78, n° 4
pages 368-373 (juillet 2011)
Doi : 10.1016/j.jbspin.2010.10.004
accepted : 7 October 2010
Ghrelin gene polymorphisms in rheumatoid arthritis
 

Metin Ozgen a, Suleyman Serdar Koca a, , Ebru Onalan Etem b, Huseyin Yuce b, Suleyman Aydin c, Ahmet Isik a
a Department of Rheumatology, Faculty of Medicine, Firat University, Elazig, Turkey 
b Department of Medical Biology and Genetics, Faculty of Medicine, Firat University, Elazig, Turkey 
c Department of Biochemistry, Faculty of Medicine, Firat University, Elazig, Turkey 

Corresponding author. Tel.: +90 424 2333555; fax: +90 424 2388096. Firat Universitesi, Tip Fakultesi, Romatoloji BD., 23119-Elazig, Turkey.
Abstract
Objectives

Ghrelin, an endogenous orexigenic peptide, has anti-inflammatory effects, down-regulates pro-inflammatory cytokines, and its altered levels are reported in various inflammatory diseases. The human preproghrelin (ghrelin/obestatin) gene shows several single nucleotide polymorphisms (SNPs) including Arg51Gln, Leu72Met, Gln90Leu, and A-501C. The aim of this study was to investigate the frequency, and clinical significance, of these four SNPs in a small cohort of Turkish patients with rheumatoid arthritis (RA).

Methods

The study included 103 patients with RA and 103 healthy controls. In the RA group, disease activity and disease-related damage were assessed using the Disease Activity Score-28 (DAS-28), and the modified Larsen scoring (MLS) methods. In all the participants, genomic DNA was isolated and genotyped by polymerase chain reaction and restriction fragment length polymorphism analysis.

Results

The frequencies of ghrelin gene SNPs were 82.5 and 79.6% in the RA and control groups, respectively, and there were no significant differences in terms of genotype distributions and allele frequencies for these four SNPs between the groups. However, the A-501C SNP was found to be associated with early disease onset, and Gln90Leu SNP with less frequent rheumatoid factor positivity, in the RA group.

Conclusion

A-501C SNP is associated with earlier onset of RA suggesting that genetic variations in the ghrelin gene may have an impact on RA.

The full text of this article is available in PDF format.

Keywords : Ghrelin gene polymorphisms, Inflammation, Rheumatoid arthritis




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